CSD Method

The CSD method allows the rapid detection of Cronobacter spp in human food products (especially milk powders and infant ingredients) and products from the production environment.

At the end of an enrichment phase with Salmonella Enrichment + CSD supplement, a subculture is carried out on CCI agar. The characteristic colonies will be confirmed.

The CSD method is NF VALIDATION certified, for the detection of Cronobacter spp, according to the validation protocol NF EN ISO 16140-2 of 2016, for the following categories:

– Infant milk powders, with and without probiotics; ingredients for test intakes from 0 to 50 g, with a 1/10th dilution.

– Infant milk powders, with and without probiotics; ingredients for test intakes from 50 to 375 g, with a ¼ dilution.

– Samples of the production environment

Refer to the certificate available on the NF VALIDATION website for the end of validity date of the method.

The reference method used for the validation is the standard EN ISO 22964 : 2017.

The CCI agar complies with the formulation described in the ISO 22964 standard of 2017.

The CSD supplement allows the common enrichment of Salmonella and Cronobacter in infant milk powders with and without probiotics, as well as ingredients and environmental products.

For the detection of Salmonella spp, refer to the IRIS method, BKR 23/07-10/11, certified NF VALIDATION.