TGEA Medium (Tryptone Glucose Yeast Extract Agar)
Principles and uses
TGEA Medium (Tryptone Glucose Yeast Extract Agar) is a medium used for the total count of aerobic mesophilic bacteria in water and dairy products. Casein Peptone and Beed Extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is source of vitamins, particularly the B-group. Dextrose is the fermentable carbohydrate providing carbon and energy. Bacteriological agar is the solidifying agent
Preparation
Suspend 28 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes
For total count of aerobics organisms in water, dairy products, etc.
Glucose 1 Bacteriological agar 18 Beef extract 3 Casein peptone 5 Yeast extract 1
