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MMGM (Minerals Modified Glutamate medium) Broth -image

MMGM (Minerals Modified Glutamate medium) Broth 

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MMGM (Minerals Modified Glutamate medium) Broth 

Minerals Modified Glutamate Broth (MMBG) ISO is recommended by ISO 16649-3 for enumeration of E.coli using the most probable number Method (MPN). MMGB Broth is also an alternative broth used for the presumptive identification of coliforms in water.
In comparison to other media, this broth is better as it gives fewer false positive results. Sodium glutamate and sodium formate are the basis of the medium for the enumeration of coliform organisms in water. Lactose is the source of carbohydrates. The addition of vitamins, aminoacids and heptahydrate magnesium sulfate increase fermentation, whereas the addition of ferric ammonium citrate permits increase of gas production. The bromocresol purple is a pH indicator.

Medium for enumerating coliform organisms in water.

Bromocresol purple 0,01 Dipotassium phosphate 0,9 Ferric ammonium citrate 0,01 Lactose 10 L-Arginine 0,02 L-Cystine 0,02 Magnesium sulfate heptahydrated 0,1 Thiamine 0,001 Sodium glutamate 6,35 Sodium formate 0,25 L (-) aspartic acid 0,024 Calcium chloride dihydrate 0,01 Panthothenic acid 0,001 Nicotinic acid 0,001