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For the identification and enumeration of Pseudomonas aeruginosa by membrane filtration

Pseudomonas CN Agar Base is used for the identification of Pseudomonas aeruginosa by membrane filtration technique, based on the detection of pyocyanin production. It is a modification of Pseudomonas P Agar (King A Medium ). This medium is recommended by ISO 16266. Pseudomonas aeruginosa is an opportunist pathogen for humans, capable of growing in water with a low concentration of nutrients. This is why natural mineral water and spring water should be checked to be free of Pseudomonas aeruginosa at the time of commercialization. This microorganism can also be found in swimming pool water.
Peptone and casein provide nitrogen, vitamins, minerals and amino acids essential for growth. Cetrimide is added as a selective agent, and nalidixic acid to suppress contaminants of cetrimide media such as Klebsiella, Proteus and Providencia spp. Potassium sulfate and magnesium chloride provide cations to activate pyocyanin production and enhance pigment production. Bacteriological agar is the solidifying agent.