Glucose Salt Teepol Broth
DESCRIPTION
Glucose Salt Teepol Broth is a selective enrichment medium used for the enumeration of V. parahemolyticus from seafoods by the most-probable-number (MPN) technique.
PRINCIPLE
Peptone and meat extract provide amino acids, nitrogen, carbon, minerals, vitamins and minerals which support the growth of
microorganism. Glucose is the fermentable carbohydrate. The high concentration of sodium chloride helps for the better enrichment of halophilic V. parahemolyticus. Methyl violet is the pH indicator.
Supplementation with Teepol serves to inhibit the growth of gram-positive organisms.
PREPARATION
Suspend 48.0 g of powder in 1 liter of deionized or distilled water. Add Teepol to obtain a concentration of 4 g/l in the complete medium. Mix well. Sterilize by autoclaving at 121°C for 15 minutes. Distribute into final containers.