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For the confirmation of coliforms in water and food samples.

Blood Agar Base is used for the isolation, cultivation and detection of hemolytic reaction of fastidious microorganisms.
It is suitable for isolating and cultivating a wide range of microorganisms with difficult growth characteristics. Upon adding blood, it can be utilized for determining hemolytic reactions.
The heart infusion and meat peptone are rich sources of nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent.
The addition of blood provides extra growth factors for fastidious microorganisms and is the basis for determining hemolytic reactions. Hemolytic patterns may vary with the type of blood or base medium used. For example, defibrinated sheep blood gives best results for Group A streptococci.

Coliforms Confirmation

For clinical diagnosis, the type of sample is secretions of the respiratory tract. - Use standard procedures to obtain isolated colonies from specimens. - Incubate at 35±2 ºC for 24-48 hours. - Since many pathogens require carbon dioxide on primary isolation, plates may be incubated in an atmosphere containing approximately 5-10% CO2. Results: 1. Alpha-hemolysis: greenish discoloration of medium. 2. Beta-hemolysis: clear zone surrounding colony. 3. Gamma-hemolysis: no change.

ISO 4831 ISO 4832

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