SIM Agar
| Formula | g/l |
| Casein Peptone | 20.0 |
| Meat Peptone | 6.1 |
| Ferric Ammonium Sulphate | 0.2 |
| Sodium Thiosulphate | 0.2 |
| Agar | 3.5 |
| Final pH 7.3 ± 0.2 at 25°C | |
Preparation
# Suspend 30.0 g of the powder in 1 liter of distilled or deionized water.
# Mix well. Heat to boil and shake until completely dissolved. Dispense into tubes.
# Sterilize in autoclave at 121°C for 15 minutes.
Quality control (35 ± 2°C / 18-24 h)
| Organism | Result |
| Escherichia coli ATCC® 25922 Salmonella Typhimurium ATCC® 14028 Shigella flexneri ATCC® 12022 | Growth Good, H2S (-), Indole (+), Motility (+) Growth Good, H2S (+), Indole (-), Motility (+) Growth Good, H2S (-), Indole (-), Motility (-) |
Storage
Store product in the original container with the lid tightly closed at between 10 and 30°C in low humidity conditions away from direct sunlight. Kept under these conditions, the product may be used up to the date of expiry shown on the product label.
Ordering information
| Code | Name | Package |
| SIM500 | AgarCult SIM Agar | 500g/box |
Semisolid medium for the identification of Enterobacteriaceae by sulphide, indole and motility tests.
