Chromogel Salmonella RB Agar (Rambach Agar)
ChromoGel™ Salmonella RB Agar is a selective medium used for the
isolation and identification of Salmonella from food and animal feed,
water and other materials.
The agar is chromogenic, providing two chromogenic substrates – propylene glycol and X-Gal ((5-bromo-4-chloro-3-indoyl- β-D-galactopyranoside).
Certain Salmonella species act on the propylene glycol producing acid which causes the pH indicator in the media to change color, and pinkish-red or crimson colonies appear. Additionally, other members of the family Enterobacteriaceae which are positive for the enzyme β-D-galactosidase cleave the substrate X-Gal and this results in the production of blue-green colonies.
Salmonella species that are positive for the β-D-galactosidase enzyme and can produce acid from propylene glycol appear as purple-violet colonies, while species that are capable of neither appear as yellow or colorless colonies.
Application
- The detection of Salmonella in foodstuff, water, animal feed
- The differential diagnosis of Salmonella species in various samples
