E&O supply freshly harvested aseptically collected Donor Horse Blood and Sheep Blood. These blood products are oxygenated and supplied in both Bottles and Bags for convenient asceptical addition to culture media. Plasma products can be fractionated after the addition of a range of anticoagulants: Citrate, Alsevers, Sodium Oxolate, Heparin and EDTA.

All donor animal blood products are harvested from Stress Free Animals with guaranteed PCV’s and free from any microbiological inhibitors. The most common use is for the manufacture of Blood Agar Plates. Dehydrated culture media (Columbia Agar) is added to deionised water and then sterilized at 121°C. The molten agar is then cooled to 42°C, fresh Horse Blood or Sheep Blood is then added at a 5% or 7% concentration before being poured into petridishes.

Half Fraser Broth Selective Supplement

Half Fraser Broth Selective Supplement
E&O Laboratories Ltd Half Fraser Selective Supplement (LS5003) is selective supplement for the primary enrichment of Listeria spp from food and environmental samples when used with Fraser Broth.Fraser Broth is a modification of the USDA-FSIS (United States Department of Agriculture-Food Safety Inspection Service) UVM secondary enrichment broth and is based on the formula described by Fraser and Sperber. It contains ferric ammonium citrate and
lithium chloride. Blackening of the medium is presumptive evidence of the presence of Listeria. Contrary to early indications, cultures which do not blacken cannot be assumed to be Listeria-free. All Fraser Broth enrichment cultures should be subcultured to plating medium. Fraser Broth has proven to be remarkably accurate in
detecting Listeria spp. in food and environmental samples.
All Listeria spp. hydrolyse aesculin to aesculetin. Aesculetin reacts with ferric ions which results in blackening. Another possible advantage to the addition of ferric ammonium citrate is that it has been shown that ferric ions enhance the growth of Listeria monocytogenes. Lithium chloride is included in the medium to inhibit the growth of enterococci which can also hydrolyse aesculin.
Half Fraser Broth is a modification of Fraser Broth which contains half of the concentration of nalidixic acid and acriflavine hydrochloride to aid in the recovery of stressed cells. Half Fraser Broth is used as the primary enrichment broth in the ISO methodology for the
detection of Listeria.

Liên hệ mua hàng:

Công ty Labcare Việt Nam – chuyên cung cấp, phân phối Môi trường nuôi cấy vi sinh, test sinh hóa, kháng huyết thanh, hóa chất, dụng cụ, thiết bị, vật tư tiêu hao.

Hotline: 0938976508

Email: info@labcare.vn

Website: labcare.vn

Next Product

Môi trường nuôi cấy vi sinh PALCAM Agar Base

Previous Product

Fraser Broth Selective Supplement