Môi trường nuôi cấy vi sinh Alphachem

ChromoGel Urine Agar is a chromogenic medium used for the cultivation and
isolation of pathogen bactreria from urine samples

o Very good productivity obtained with selected and standardized peptones.
o Optimized agar concentration to inhibit the swarming of Proteus spp.
o Enhanced visual differentiation of the colonies due to strong chromatic reactions and to opaque contrasting background.
o Specific enzymatic reactions for presumptive identification of both gram positive and gram negative pathogens.

The medium use for isolation and identification of Streptococcus B group
bacteria (Opaque background)

The Group B Streptococci (GBS), also known under the name of Streptococcus agalactiae, are the cause of numerous infections in adults but mostly an important cause of serious neonatal infections, occuring in the three first weeks of life. Studies indicate that approximately 12-27 % of pregnant women are colonised by GBS. (WHO, Infectious diseases, Group B Streptococcus). Detecting vaginal (and in some countries also rectal) colonisation by GBS in pregnant women is the most effective strategy to prevent infection transmission during baby delivery.

Worldwide, official guidelines recommend prenatal screening of GBS in the last month of pregnancy. In GBS-carrying women, this screening allows determining the need of intrapartum antibiotic prophylaxy, which has been proved effective in preventing the infections occuring in the first week of life (known as early-onset GBS infections).

ChromoGel™ Salmonella RB Agar is a selective medium used for the
isolation and identification of Salmonella from food and animal feed,
water and other materials.

The agar is chromogenic, providing two chromogenic substrates – propylene glycol and X-Gal ((5-bromo-4-chloro-3-indoyl- β-D-galactopyranoside).
Certain Salmonella species act on the propylene glycol producing acid which causes the pH indicator in the media to change color, and pinkish-red or crimson colonies appear. Additionally, other members of the family Enterobacteriaceae which are positive for the enzyme β-D-galactosidase cleave the substrate X-Gal and this results in the production of blue-green colonies.
Salmonella species that are positive for the β-D-galactosidase enzyme and can produce acid from propylene glycol appear as purple-violet colonies, while species that are capable of neither appear as yellow or colorless colonies.

Application

• The detection of Salmonella in foodstuff, water, animal feed
• The differential diagnosis of Salmonella species in various samples

For detection and isolation of Staphylococci by membrane filter technique.

Glucose agar allows the demonstration of glucose fermentation (with or without gas production) as an identification test for Enterobacteriaceae or Pseudomonas spp..

Drigalski Lactose agar is used for the selective isolation of Enterobacteriaceae. It allows the differentiation of bacteria according to their ability to ferment lactose in food, pharmaceutical, cosmetic and veterinary samples.

Modified Laurylsulfate-Tryptose Vancomycin broth (mLST/V) is a selective enrichment medium used for the detection of Cronobacter sakazakii in milk and milk products.

Cronobacter Screening broth is a selective enrichment media used for the detection of Cronobacter spp. in food products and ingredients intended for human consumption and the feeding of animals. It is also used for the control of production environmental samples.
The typical composition responds to that defined in the standard ISO 22964.

CCI Agar is used for the detection of microorganisms in food products and ingredients intended for human consumption and the feeding of animals.

Violet Red Bile Agar (VRBL) is a selective medium used for the detection and enumeration of coliform and thermotolerant coliforms food products.

Thioglycolate Medium with Resazurin is used for sterility tests of biological products

Preparation

# Suspend 30.0 g of the powder in 1 liter of distilled or deionized water.

# Mix well. Heat to boil and shake until completely dissolved. Dispense into tubes.

# Sterilize in autoclave at 121°C for 15 minutes.

Quality control (35 ± 2°C / 18-24 h)

Storage

Store product in the original container with the lid tightly closed at between 10 and 30°C in low humidity conditions away from direct sunlight. Kept under these conditions, the product may be used up to the date of expiry shown on the product label.

Ordering information

The Iron Sulfite Agar (ISA) (ISO 15213-1) is recommended for the enumeration of sulfite-reducing Clostridium spp.

Meat-Liver Glucose 0.6% Agar is suitable for sterility tests of pharmaceutical products and canned foods

E.E. Broth is recommended as an enrichment medium when examining food and feedstuffs for Enterobacteriaceae.
It is a modification of Brilliant Green Bile Broth, with an improved buffering capacity to encourage early growth and prevent autosterilization.

E.E. Broth uses glucose instead of lactose to make the medium a test for all enterobacteria including non-lactose fermenting organisms.
This formulation complies with the Harmonized USP/EP/JP.

Nitrogen is supplied by the gelatin peptone whilst glucose serves as the fermentable carbohydrate source.
Oxbile and brilliant green are the selective agents helping to suppress Gram-positive non- target organisms.
Auto sterilisation is prevented through the buffer system composed of potassium dihydrogen phosphate and disodium hydrogen phosphate.

Để được tư vấn chi tiết, giải đáp thắc mắc, hướng dẫn sử dụng và báo giá cụ thể, hãy liên hệ ngay với Labcare VN – Công ty TNHH Labcare Việt Nam qua các thông tin bên dưới:

• Email: info@labcare.vn
• Hotline: 0938976508
• Website: http://labcare.vn | http://moitruongvisinh.vn

A general purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms. Tryptone and soy peptone are the nitrogen and vitamin source in the medium. Glucose is the carbon energy source that facilitates organism growth and sodium chloride maintains osmotic balance. Di-potassium hydrogen phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms.
Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB. TSB conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).

Để được tư vấn chi tiết, giải đáp thắc mắc, hướng dẫn sử dụng và báo giá cụ thể, hãy liên hệ ngay với Labcare VN – Công ty TNHH Labcare Việt Nam qua các thông tin bên dưới:

• Email: info@labcare.vn
• Hotline: 0938976508
• Website: http://labcare.vn | http://moitruongvisinh.vn

Kligler iron agar is used to differentiate between some of the enterobacteriacae on the basis of three reactions: fermentation of lactose and glucose and the production of hydrogen sulphide.
Kligler iron agar is a modification of the original formulation developed by Kligler.
It incorporates the principles of Russell’s double sugar agar with Kligler ‘s lead acetate agar.
The peptone provides the required nitrogen, carbon and vitamins. Lactose and glucose are carbohydrates.
Acid production from their fermentation is detected by the phenol red pH indictor. Sodium thiosulphate is reduced to hydrogen sulphide which is detected by the ferric citrate indicator.
Sodium chloride maintains the osmotic balance.

Liên hệ mua hàng:

Công ty Labcare Việt Nam – chuyên cung cấp, phân phối Môi trường nuôi cấy vi sinh, test sinh hóa, kháng huyết thanh, hóa chất, dụng cụ, thiết bị, vật tư tiêu hao.

Hotline: 0938976508

Email: info@labcare.vn

Website: labcare.vn

Slanetz and Bartley Agar is a selective medium used for the enumeration of intestinal enterococci in drinking water, beverages, waste water, swimming pool water and various biological products of animal origin, by the membrane filtration method. 

Ethyl Violet Azide Broth (also known as Litsky broth) is used to carry out the confirmation test of the detection and enumeration of fecal streptococci (enterococci) in drinking water and waste water, in frozen foods and other food products by the most probable number method. The procedure involves two stages : 
      - presumptive test in Azide Dextrose Broth (Rothe)
      - confirmation in Ethyl Violet Azide Broth (Litsky)

Bile Esculin Azide Agar (BEA agar) is a selective medium used to isolate and enumerate enterococci in food and pharmaceutical products. It is also used for the enumeration of enterococci animal feed.

Azide Dextrose Broth (Rothe) is used for the enumeration of enterococci in drinking water, frozen foods and other food products by the most probable number method

Tryptone-Soy Agar, used as a base to be supplemented with blood, is prepared with selected starting materials which do not turn brown. It was specially designed to detect beta-hemolytic reactions and to favor the growth of particularly fastidious aerobic and anaerobic bacteria. 
The media can be used for the hemolysis test on presumed colonies of Bacillus cereus, according to the standard ISO 21871.

BCP Dextrose Tryptone Agar (also known as BCP Glucose agar) is used to enumerate mesophilic and thermophilic aerobic bacterial spores (especially Bacillus stearothermophilus, responsible for flat sour) in raw materials and in ingredients used in canning non-acid products (pH > 4,5). It is also used in surface samples and canning process water.

Bacillus cereus Agar is used for the detection and enumeration of spores and vegetative cells of Bacillus cereus in food products.
The typical composition corresponds to the MYP agar (mannitol, egg yolk, polymyxin agar) described in the standards NF EN ISO 7932 and NF EN ISO 21871.

Tryptone Sulfite Cycloserine Agar was described by Harmon for the selective isolation and enumeration of Clostridium perfringens in water and food samples. The medium was recommended for the enumeration of sulfur-reducing anaerobes from foods of animal origin.

Lactose-Sulfite Broth is a confirmation medium allowing the selective detection of both the vegetative cells and spores of Clostridium perfringens in food products and biological samples of animal origin, without the usual confirmatory tests.

Bryant and Burkey Broth with Resazurin (modified by Bergère) is used to enumerate the spores of lactate fermenting Clostridium in milk and dairy products. In particular, it is used to detect Clostridium tyrobutyricum, responsible for the late blowing of cheeses such as gruyère, emmental, gouda, edam, cheddar or parmesan. The phenomenon arises from a high number of spores in the milk used to prepare the cheese. This number depends above all on the type of food given to the animals, since the main source of contamination is silage. 

Reinforced Clostridial Agar is a non-selective medium used for the isolation and enumeration of Clostridia, other anaerobic bacteria and lactobacilli in biological samples, dairy products, and other food products.

Bromocresol Purple (BCP) Lactose Agar is a non-selective medium, used for the differentiation of Enterobacteriaceae. It is used for the confirmation of coliforms in the context of the standard T90-425, for the bacteriological examination of containers and recipients used in water bottling.  

Tryptophan broth allows the culture of microorganisms presenting no particular growth requirements. The media is used to perform the indole production test, used notably in the confirmation of Escherichia coli, Shigella and Salmonella.

Glutamate broth is used enumeration of β-glucuronidase positive E. coli in food products by the MPN technique. It is recommended when the microorganisms are found in a sub-optimal state due to exposition to stress including freezing, desiccation, disinfection or high salinity.
The described method does not allow the detection of strains of Escherichia coli that do not develop at 44°C, and, in particular, those that are β-glucuronidase negative, as the case of E. coli O157 and a few other strains of pathogenic E. coli.

EMB Agar, originally recommended by Levine, is used to isolate and identify enterobacteria (notably Escherichia coli and Enterobacter aerogenes) in pharmaceutical, cosmetic and food products as well as water.
It is also used as a confirmation media for Escherichia coli in cosmetic products.

EC Broth (abbreviation for Escherichia coli) is a selective media used for the confirmation of E. coli in water, milk and other food products, as well as live shellfish.
The typical composition of the broth responds to that defined in the normalized method of presumptive Escherichia coli enumeration.

Laurylsulfate-Tryptose broth is a selective enrichment media used for the detection and enumeration of Escherichia coli and coliforms in water and food products. 
The medium was formulated by Malmann and Darby who showed, in 1941, that sodium laurylsulfate was the best selective agent among a number of surfactants and did not inhibit the growth of coliform bacteria. Levine later showed that the medium reduced the number of false positives by inhibiting the growth of sporulated gas-producing bacteria.

Lactose Broth is used principally as a liquid enrichment media for the detection of Escherichia coli in cosmetic products, if neutralizers or dispersal agents are not necessary. It is also adapted to the culture of all Gram negative bacteria. Armed with a Durham tube, it can detect the fermentation of lactose.

Principles and uses
Endo Agar Base is a differential and moderately selective culture medium for the detection and confirmation of coliforms and other enteric microorganisms in waters, milk, dairy and other food products.
It uses fuchsin to differentiate between positive lactose-fermenting and lactose non-fermenting bacteria. Acetaldehyde production by lactose-fermenting organisms such as E. coli produce characteristic red colonies and a red surrounding area, marked by its reaction with Sodium sulphite in the presence of fuchsin. Lactose non-fermenters form colorless, transparent colonies.
Peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Lactose is the fermentable carbohydrate providing carbon and energy. Dipotassium phosphate acts as a buffer system. Bacteriological agar is the solidifying agent.

Formula:

For 1 liter of media :
- Pancreatic digest of meat.......................................................................................... 10,0 g
- Lactose...................................................................................................................... 10,0 g
- Dipotassium phosphate............................................................................................... 3,5 g
- Sodium sulfite.............................................................................................................. 2,5 g
- Basic fuchsin .............................................................................................................. 0,5 g
- Bacteriological agar................................................................................................... 15,0 g
pH of the ready-to-use media at 25 °C : 7,5 ± 0,2. 

Preparation
Dissolve 41,5 g of dehydrated media (BK057) in 1 liter of distilled or demineralized water.
 Slowly bring to boiling, stirring with constant agitation until complete dissolution.
 Dispense in tubes or flasks.
 Sterilize in an autoclave at 121 °C for 15 minutes.
 Cool and maintain in a molten state at 44-47 °C.
 Mix well in order to distribute any precipitate.
 Pour into sterile Petri plates and let solidify on a cool, even surface.
 Dry the plates in an incubator, covers partially removed.

Desoxycholate (0.1%) Lactose Agar is a selective medium for the enumeration of coliform bacteria in milk, dairy products and other food products. The medium is also recommended for the isolation and culture of Shigella.

Bromocresol Purple (BCP) Lactose broth is used as enrichment media for the enumeration of coliforms and of thermotolerant coliforms in water

Brilliant Green Bile Broth (BGBB) is used for the confirmation of coliforms and thermotolerant coliforms in food products, water used for watering food products and for effluent / run-off water testing. It can also be used as an enumeration media for coliforms in frozen dairy and ice cream products. 

TSI (Triple Sugar Iron) Agar is used for the identification of enterobacteria by the rapid detection of the fermentation of lactose, glucose (with or without gas production) and of sucrose, as well as the production of hydrogen sulfide.
The typical composition corresponds to that defined in the standards NF EN ISO 6579-1 and NF EN ISO 19250 for the detection of Salmonella spp..

Tryptophan broth allows the culture of microorganisms presenting no particular growth requirements. The media is used to perform the indole production test, used notably in the confirmation of Escherichia coli, Shigella and Salmonella.