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ChromoGel Urine Agar is a chromogenic medium used for the cultivation and
isolation of pathogen bactreria from urine samples

o Very good productivity obtained with selected and standardized peptones.
o Optimized agar concentration to inhibit the swarming of Proteus spp.
o Enhanced visual differentiation of the colonies due to strong chromatic reactions and to opaque contrasting background.
o Specific enzymatic reactions for presumptive identification of both gram positive and gram negative pathogens.

The medium use for isolation and identification of Streptococcus B group
bacteria (Opaque background)

The Group B Streptococci (GBS), also known under the name of Streptococcus agalactiae, are the cause of numerous infections in adults but mostly an important cause of serious neonatal infections, occuring in the three first weeks of life. Studies indicate that approximately 12-27 % of pregnant women are colonised by GBS. (WHO, Infectious diseases, Group B Streptococcus). Detecting vaginal (and in some countries also rectal) colonisation by GBS in pregnant women is the most effective strategy to prevent infection transmission during baby delivery.

Worldwide, official guidelines recommend prenatal screening of GBS in the last month of pregnancy. In GBS-carrying women, this screening allows determining the need of intrapartum antibiotic prophylaxy, which has been proved effective in preventing the infections occuring in the first week of life (known as early-onset GBS infections).

ChromoGel™ Salmonella RB Agar is a selective medium used for the
isolation and identification of Salmonella from food and animal feed,
water and other materials.

The agar is chromogenic, providing two chromogenic substrates – propylene glycol and X-Gal ((5-bromo-4-chloro-3-indoyl- β-D-galactopyranoside).
Certain Salmonella species act on the propylene glycol producing acid which causes the pH indicator in the media to change color, and pinkish-red or crimson colonies appear. Additionally, other members of the family Enterobacteriaceae which are positive for the enzyme β-D-galactosidase cleave the substrate X-Gal and this results in the production of blue-green colonies.
Salmonella species that are positive for the β-D-galactosidase enzyme and can produce acid from propylene glycol appear as purple-violet colonies, while species that are capable of neither appear as yellow or colorless colonies.

Application

• The detection of Salmonella in foodstuff, water, animal feed
• The differential diagnosis of Salmonella species in various samples

For detection and isolation of Staphylococci by membrane filter technique.

Glucose agar allows the demonstration of glucose fermentation (with or without gas production) as an identification test for Enterobacteriaceae or Pseudomonas spp..

Drigalski Lactose agar is used for the selective isolation of Enterobacteriaceae. It allows the differentiation of bacteria according to their ability to ferment lactose in food, pharmaceutical, cosmetic and veterinary samples.

Modified Laurylsulfate-Tryptose Vancomycin broth (mLST/V) is a selective enrichment medium used for the detection of Cronobacter sakazakii in milk and milk products.

Cronobacter Screening broth is a selective enrichment media used for the detection of Cronobacter spp. in food products and ingredients intended for human consumption and the feeding of animals. It is also used for the control of production environmental samples.
The typical composition responds to that defined in the standard ISO 22964.

CCI Agar is used for the detection of microorganisms in food products and ingredients intended for human consumption and the feeding of animals.

Violet Red Bile Agar (VRBL) is a selective medium used for the detection and enumeration of coliform and thermotolerant coliforms food products.

Thioglycolate Medium with Resazurin is used for sterility tests of biological products

Preparation

# Suspend 30.0 g of the powder in 1 liter of distilled or deionized water.

# Mix well. Heat to boil and shake until completely dissolved. Dispense into tubes.

# Sterilize in autoclave at 121°C for 15 minutes.

Quality control (35 ± 2°C / 18-24 h)

Storage

Store product in the original container with the lid tightly closed at between 10 and 30°C in low humidity conditions away from direct sunlight. Kept under these conditions, the product may be used up to the date of expiry shown on the product label.

Ordering information

The Iron Sulfite Agar (ISA) (ISO 15213-1) is recommended for the enumeration of sulfite-reducing Clostridium spp.

Meat-Liver Glucose 0.6% Agar is suitable for sterility tests of pharmaceutical products and canned foods