Danh mục
Môi trường nuôi cấy vi sinh Baird Parker Agar
Baird Parker agar is a selective medium for the isolation and presumptive identification of coagulase-positive staphylococci. This medium is used extensively for detecting Staphylococcus aureus in foods, dairy products, and other materials.
Enzymatic digest of casein, meat extract and yeast extract provide the required carbon, nitrogen and vitamins. The medium is made selective by the inclusion of lithium chloride and the addition of potassium tellurite. Glycine and sodium pyruvate are included as growth enhancers while the pyruvate also acts as a neutraliser of toxic peroxides.
Coagulase positive staphylococci are differentiated by the addition of egg yolk tellurite (BM0530) due to their ability to break down the egg yolk. Clear zones around colonies form due to the
proteolytic action of lecithinase. A secondary opaque zone,
surrounding presumptive positive colonies, may also form due to lipase activity. Potassium tellurite is reduced by staphylococci giving rise to blackening of colonies.
NB: Any black colonies (with halo (typical) or without the halo (atypical)) on this medium must be confirmed as Staphylococcus aureus by further tests (e.g. coagulase test or latex agglutination etc.)
Related Supplements :
BM0530 Egg Yolk Tellurite
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