E&O Laboratories Ltd have successfully grown their Culture Media business over the last 30 years to become a leading manufacturer
servicing Microbiology Laboratories , Location in Scotland. Their comprehensive media range is continually expanding into specialised industry sectors as well as aiding the rapid diagnosis of the newest antibiotic resistant bacteria strains.
INDUSTRY SECTORS:
• Clinical, Veterinary & Aquaculture
• Food, Water & Environmental
• Pharmaceutical, Industrial & Cosmetics
• Academia & Research
PRODUCT CATEGORIES:
• Ready-to-use Culture Media – Bottled, Bagged and Plated
• Dehydrated Culture Media & Raw Materials
• Antibiotic Supplements & Reagents
• Fresh Donor Horse & Sheep Blood – Frozen Sterile Filtered Serum
Dehydrated Culture Media products are formulated to supply the required nutrients to allow for the growth of microorganisms. Used in combination with a variety of selective agents and incubation conditions a wide range of specific organisms can be isolated. With careful raw material selection of the various media components E&O can ensure a consistent level of quality and performance. For each formulation the necessary ingredients are accurately weighed, combined and blended together to produce a homogenous powdered product.
Nitrate broth is a liquid medium recommended to differentiate microorganisms based on their ability to reduce nitrate to nitrite (denitrification). Members of the Enterobacteriaceae family use nitrate as the source of nitrogen, but it must be broken down to be utilized. Depending on the bacterial species, the final product will be nitrite, ammonia or nitrogen gas. Potassium Nitrate is the nitrogen source in this medium. Nitrate breakdown to nitrite is detected by adding Sulfanilic Acid, which forms a diazonium salt when reacting with nitrite, and N,N-dimethyl-α-naphtylamine, which forms a red complex with the diazonium salt. To detect microorganisms converting nitrate to ammonia or nitrogen gas, zinc dust must be added. Zinc reduces nitrate to nitrite, resulting in a red color. While the presence of red color indicates that nitrate is still present, its absence evidences nitrate breakdown further than nitrate by the bacteria.
Phenol Red Sucrose Broth is a liquid medium recommended to differentiate microorganisms based on their ability to ferment sucrose. Proteose Peptone and Beef Extract provide nitrogenous compounds, carbon, vitamins and trace elements to the microorganisms, while Sodium Chloride serves as source of essential electrolytes and maintains the osmotic equilibrium. All members of the Enterobacteriaceae family can use Sucrose as an energy source, and therefore grow well in this medium. Sucrose fermentation produces gases and organic acids, being butyric acid the main constituent. The pH indicator Phenol Red shifts from red to yellow upon accumulation of these products, thus revealing Sucrose fermentation. Additionally, production of butyric acid can be recognized by a pungent, foul odor.
Tryptone-Soy Agar, used as a base to be supplemented with blood, is prepared with selected starting materials which do not turn brown. It was specially designed to detect beta-hemolytic reactions and to favor the growth of particularly fastidious aerobic and anaerobic bacteria.
The media can be used for the hemolysis test on presumed colonies of Bacillus cereus, according to the standard ISO 21871.
BCP Dextrose Tryptone Agar (also known as BCP Glucose agar) is used to enumerate mesophilic and thermophilic aerobic bacterial spores (especially Bacillus stearothermophilus, responsible for flat sour) in raw materials and in ingredients used in canning non-acid products (pH > 4,5). It is also used in surface samples and canning process water.
