Môi trường vi sinh EOLabs

E&O Laboratories Ltd have successfully grown their Culture Media business over the last 30 years to become a leading manufacturer
servicing Microbiology Laboratories , Location in Scotland. Their comprehensive media range is continually expanding into specialised industry sectors as well as aiding the rapid diagnosis of the newest antibiotic resistant bacteria strains.

INDUSTRY SECTORS:
• Clinical, Veterinary & Aquaculture
• Food, Water & Environmental
• Pharmaceutical, Industrial & Cosmetics
• Academia & Research

PRODUCT CATEGORIES:
• Ready-to-use Culture Media – Bottled, Bagged and Plated
• Dehydrated Culture Media & Raw Materials
• Antibiotic Supplements & Reagents
• Fresh Donor Horse & Sheep Blood – Frozen Sterile Filtered Serum

Dehydrated Culture Media products are formulated to supply the required nutrients to allow for the growth of microorganisms. Used in combination with a variety of selective agents and incubation conditions a wide range of specific organisms can be isolated. With careful raw material selection of the various media components E&O can ensure a consistent level of quality and performance. For each formulation the necessary ingredients are accurately weighed, combined and blended together to produce a homogenous powdered product.

Môi trường vi sinh Alphachem

Môi trường vi sinh Thiosulfate Citrate Bile Saccharose (TCBS) Agar

Môi trường vi sinh ALKALINE SALINE PEPTONE WATER

Môi trường vi sinh Alphachem

Môi trường vi sinh Thiosulfate Citrate Bile Saccharose (TCBS) Agar

Principles and uses
TCBS Agar is a selective medium widely used to isolate and cultivate practically all bacteria of the genus Vibrio, including V. cholerae and V. alginolyticus, pathogenic to humans causing cholera, choleral diarrhea or food poisoning from contaminated foods and from stool specimens. The last 2
conditions especially can be caused by ingesting raw or partially processed fish or seafood containing Vibrio parahemolyticus. The only Vibrio that does not grow in TCBS is V. hollisae.
The meat and casein peptones provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Sodium citrate, sodium thiosulfate and ox bile are the selective agents, inhibiting the Gram positive bacteria. Sodium
thiosulfate provides sulphur, and ferric citrate is the indicator for H2S production. Sucrose is the carbohydrate energy source. Bromothymol blue and thymol blue are pH indicators. Sodium chloride promotes growth (Vibrio grows well in salty media). Bacteriological agar is the solidifying agent. The alkaline pH of the medium enhances the recovery of V. cholerae.
Sucrose-positive vibrios, such as Vibrio cholerae and Vibrio alginolyticus, are yellow on TCBS. Sucrose-negative ones, such as Vibrio parahaemolyticus and Vibrio vulnificus, produce blue- green colonies. Almost all Vibrio ferment sucrose and yield yellow colonies from the production of acid. Some types of Proteus (fermenters of sucrose) can form yellow colonies similar to those of Vibrio.

Preparation
Suspend 88 grams of the medium in one liter of distilled water. Mix well. Dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. DO NOT OVEARHEAT. DO NOT AUTOCLAVE. Cool to 50 ºC, mix well and dispense into plates.

Môi trường vi sinh ALKALINE SALINE PEPTONE WATER

Alkaline saline peptone water (ASPW) is used as a primary and secondary enrichment medium for the detection of Vibrio spp. in human and animal food products as well as environmental samples.
The typical composition meets food microbiology standards XP ISO/TS 21872-1 and XP ISO/TS 21872-2.

Môi trường vi sinh Alphachem

Môi trường vi sinh Thiosulfate Citrate Bile Saccharose (TCBS) Agar

Principles and usesTCBS Agar is a selective medium widely used to isolate and cultivate practically all bacteria of the genus Vibrio, including V. cholerae and V. alginolyticus, pathogenic to humans causing cholera, choleral diarrhea or food poisoning from contaminated foods and from stool specimens. The last 2conditions especially can be caused by ingesting raw or partially processed fish or seafood containing Vibrio parahemolyticus. The only Vibrio that does not grow in TCBS is V. hollisae.The meat and casein peptones provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Sodium citrate, sodium thiosulfate and ox bile are the selective agents, inhibiting the Gram positive bacteria. Sodiumthiosulfate provides sulphur, and ferric citrate is the indicator for H2S production. Sucrose is the carbohydrate energy source. Bromothymol blue and thymol blue are pH indicators. Sodium chloride promotes growth (Vibrio grows well in salty media). Bacteriological agar is the solidifying agent. The alkaline pH of the medium enhances the recovery of V. cholerae.Sucrose-positive vibrios, such as Vibrio cholerae and Vibrio alginolyticus, are yellow on TCBS. Sucrose-negative ones, such as Vibrio parahaemolyticus and Vibrio vulnificus, produce blue- green colonies. Almost all Vibrio ferment sucrose and yield yellow colonies from the production of acid. Some types of Proteus (fermenters of sucrose) can form yellow colonies similar to those of Vibrio. PreparationSuspend 88 grams of the medium in one liter of distilled water. Mix well. Dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. DO NOT OVEARHEAT. DO NOT AUTOCLAVE. Cool to 50 ºC, mix well and dispense into plates. Read More

Môi trường vi sinh ALKALINE SALINE PEPTONE WATER

Alkaline saline peptone water (ASPW) is used as a primary and secondary enrichment medium for the detection of Vibrio spp. in human and animal food products as well as environmental samples.The typical composition meets food microbiology standards XP ISO/TS 21872-1 and XP ISO/TS 21872-2. Read More