Guaiacol Detection Kit
Detection of Alicyclobacilli spp. and Alicyclobacilli which form guaiacol
Alicyclobacilli are aerobic, acidophilic and thermophilic bacteria
that can grow in highly acidic beverages such as in fruit juices, but
also in the raw materials for these, such as fruit juice concentrates or
sugar syrup. With their ability to form spores, they can survive the
pasteurisation process and continue to grow vegetatively under optimal
conditions. According to scientific findings, only 3 of the
approximately 40 known alicyclobacilli have the potential to produce the
guaiacol which spoils beverages with its strong off-flavour (intense
chemical smell).
The detection method takes place in 2 steps.
First, the possible contamination is enriched and isolated via
cultivation with our ready-to-use nutrient media BAT Broth and Agar. In the second step, the biochemical Guaiacol Detection Kit is used to analyse the isolated alicyclobacillus bacteria for its ability to form guaiacol.
The
BAT Culture Media and the Guaiacol Detection Kit are described in the
IFU (International Fruit and Vegetable Juice Association) Method No.12
"Method on the Detection and Enumeration of Spore-forming
Thermo-Acidophilic Spoilage bacteria“