Môi trường vi sinh de Man, Rogosa & Sharpe (MRS) Agar
This medium is used for the cultivation and enumeration of Lactobacillus spp. MRS agar is based on the formulation by de Man, Rogosa and Sharpe.
The peptone, yeast extract and beef extract provides the reuired carbon, nitrogen and vitamin source. Glucose is a fermentable carbohydrate. The magnesium sulphate and manganese sulphate act as growth stimulants. Potassium phosphate is a buffering agent. Selectivity of the medium is achieved through the use of ammonium citrate and sodium acetate, inhibiting microorganisms such as streptococci and moulds. The addition of the surfactant Tween 80 is required to facilitate the uptake of nutrients from Lactobacillus spp.
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