Môi trường vi sinh Fraser Half-Broth Strength

Half-Fraser broth is a selective enrichment broth for the identification and isolation of Listeria spp., primarily from food and environmental samples.
The antibiotics, nalidixic acid and acriflavine, are already included in the formulation so only ferric ammonium citrate (LS5004) need be added to make the complete Half-Fraser broth.
The peptones provide carbon, nitrogen and vitamins, sodium chloride provides osmotic balance and the phosphate buffer system maintains pH.
Lithium chloride inhibits enterococci and the antibiotics make the medium highly selective.
Listeria spp. hydrolyse aesculin to aesculetin which forms a confirmatory dark brown or black complex with Fe3+ ions.

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