Olaquindox Metabolite (MQCA) ELISA Test Kit

Brand: FSTest

CatNO: DR32.5066.5

Specifications: 96tests/kit

Shelf life: 12 months

Application: Designed for rapid and accurate detection of 3-methylquinoxaline-2-carboxylic acid (MQCA) residues in meat (poultry, pork), fish, shrimp, and other seafood products, ensuring food safety and regulatory compliance.

FSTest Olaquindox Metabolite (MQCA) ELISA Test Kit – High-Sensitivity Detection for Banned Growth Promoter Monitoring

Introduction:

The FSTest Olaquindox Metabolite ELISA Test Kit is a highly specific immunoassay designed for quantitative detection of 3-methylquinoxaline-2-carboxylic acid (MQCA), the marker residue of banned growth promoter olaquindox, in animal tissues and seafood.     With a 0.5ppb detection limit, this kit provides reliable monitoring to comply with global food safety regulations.

Key Features & Benefits:

✔ Ultra-Sensitive Detection - 0.5ppb limit of detection for precise MQCA quantification

✔ Exceptional Specificity - 100% cross-reactivity with MQCA,<1% with olaquindox/carbadox

✔ Regulatory Compliance - Meets requirements for monitoring banned quinoxaline drugs

✔ High-Throughput Format - 96-well design for efficient batch processing

✔ Validated Performance - ≤10% CV ensures reproducible results

✔ Streamlined Workflow - Optimized extraction protocol for meat and seafood

Applications:

Monitoring banned olaquindox residues in pork, poultry

Seafood safety testing (fish, shrimp)

Food import/export compliance verification

Veterinary drug residue surveillance programs

Testing Principle:

This competitive ELISA measures absorbance at 450nm (inversely proportional to MQCA concentration) against a standard curve (0-16.2ppb range).

How to Use:

1️⃣ Sample Preparation:

Homogenize tissue/seafood samples

Extract with ethyl acetate/HCl

Evaporate and reconstitute

2️⃣ Test Procedure:

Add 50μL sample/standard to wells

Add 50μL enzyme conjugate & antibody

Incubate 30min at 20-28°C

Wash 4-5 times

Add substrate, incubate 30min

Add stop solution

Measure absorbance at 450nm using a microplate reader.

3️⃣ Result Interpretation:

Compare to standard curve (0.3-16.2ppb)

Multiply by 1.5× dilution factor

Why Choose FSTest MQCA ELISA Kit?

✅ Banned Drug Monitoring - Specifically detects MQCA marker residue

✅ Regulatory-Grade Sensitivity - 0.5ppb detection meets strictest requirements

✅ Time-Efficient - Complete results in<2.5 hours

✅ Cost-Effective - 96-well format reduces per-test cost

Technical Specifications:

Detection Limit: 0.5ppb

Accuracy: 70-130% recovery

Sample Types: Animal tissues, seafood

Format: 96-well microplate

Storage: 2-8°C (12 month shelf life)

Storage & Stability:

Store at 2-8°C. Do not freeze. Stable for 12 months.

Ensure compliance with global food safety regulations using the FSTest Olaquindox Metabolite ELISA Test Kit - the most reliable solution for MQCA residue detection.