Ractopamine ELISA Test Kit
Brand: FSTest
CatNO: DR32.5078.5
Specifications: 96tests/kit
Shelf life: 12 months
Application: Designed for quantitative detection of ractopamine residues in animal tissues and liver samples, ensuring food safety and regulatory compliance.
FSTest Ractopamine ELISA Test Kit – High-Sensitivity Detection for Banned Growth Promoters
Introduction:
The FSTest Ractopamine ELISA Test Kit is a precision immunoassay designed for quantitative detection of ractopamine residues in animal tissues and liver samples. With a 0.4ppb detection limit, this kit enforces compliance with global bans on this prohibited β-agonist growth promoter.
Key Features & Benefits:
✔ Ultra-Sensitive Detection – 0.4ppb LOD (0.1ppb LOQ) meets strictest regulatory requirements
✔ High Specificity – 100% for ractopamine,<1% cross-reactivity with other β-agonists
✔ Regulatory Compliance – Supports enforcement of EU/China bans on growth promoters
✔ Tissue-Specific Validation – Optimized for both muscle and liver matrices
✔ Efficient Workflow – 96-well format processes 20+ samples per run
✔ Ready-to-Use – Pre-coated plates and optimized reagents reduce setup time
Applications:
Meat safety monitoring (pork, beef)
Liver residue testing
Veterinary drug surveillance
Food import/export compliance
Regulatory enforcement programs
Testing Principle:
Competitive ELISA measuring absorbance at 450nm (inversely proportional to ractopamine concentration) against a 6-point standard curve (0-8.1ppb).
How to Use:
1️⃣ Sample Preparation:
Homogenize tissue/liver samples
Extract with HCl/NaCl/methanol solution
Centrifuge and pH adjust (7.8)
Dilute with sample diluent
2️⃣ Test Procedure:
Add 50μL sample/standard
Add 50μL enzyme conjugate & antibody
Incubate 30min (20-28°C)
Wash 4-5 times
Add substrate, incubate 15min
Add stop solution
Read at 450nm
3️⃣ Result Interpretation:
Compare to standard curve (0.1-8.1ppb)
Apply 4× dilution factor
Why Choose FSTest Ractopamine ELISA Kit?
✅ Banned Substance Focus – Specifically targets prohibited growth promoters
✅ Matrix-Specific Protocols – Different pH adjustment for tissue vs liver
✅ Regulatory Ready – Complies with global β-agonist bans
✅ Time-Efficient – Complete analysis in<2 hours
✅ Cost-Effective – 96 tests/kit reduces per-sample cost
Technical Specifications:
Detection Limit: 0.4ppb
LOQ: 0.1ppb
Accuracy: 70-130% recovery
Precision: ≤10% CV
Format: 96-well microplate
Storage: 2-8°C (12 months)
Regulatory Compliance:
EU Directive 96/22/EC
China Ministry of Agriculture Announcement No. 235
US FDA Compliance Policy Guide
Codex Alimentarius standards
