Malachite Green ELISA Test Kit
Brand: FSTest
CatNO: DR32.5074.5
Specifications: 96tests/kit
Shelf life: 12 months
Application: Designed for quantitative detection of malachite green (MG) and its metabolite leucomalachite green (LMG) in seafood samples, ensuring food safety and regulatory compliance.
FSTest Malachite Green ELISA Test Kit – Ultra-Sensitive Detection for Banned Aquaculture Chemicals
Introduction:
The FSTest Malachite Green ELISA Test Kit is a high-precision immunoassay designed for quantitative detection of malachite green (MG) and its metabolite leucomalachite green (LMG) in seafood samples. With an exceptional 0.15ppb detection limit, this kit enforces compliance with global bans on these carcinogenic aquaculture chemicals.
Key Features & Benefits:
✔ Ultra-Sensitive Detection – 0.15ppb LOD (0.01ppb LOQ) meets strictest regulatory requirements
✔ Dual-Analyte Coverage – Detects both MG (100%) and LMG (20%)
✔ Regulatory Compliance – Supports enforcement of global MG bans in aquaculture
✔ Specialized Seafood Testing – Optimized for fish and shrimp matrices
✔ High-Throughput Format – 96-well design for efficient batch processing
✔ Validated Performance – ≤10% CV ensures reproducible results
Applications:
Seafood safety monitoring (fish, shrimp)
Aquaculture product export testing
Food import compliance verification
Regulatory enforcement programs
Testing Principle:
Competitive ELISA measuring absorbance at 450nm (inversely proportional to MG concentration) against a 6-point standard curve (0-0.08ppb).
How to Use:
1️⃣ Sample Preparation:
Homogenize seafood samples
Extract with acetonitrile/dichloromethane
Purify with neutral alumina
Oxidize with specialized reagents (fish: 30min; shrimp: 30min)
2️⃣ Test Procedure:
Prepare fresh standard series (0-0.08ppb)
Add 100μL antibody solution, incubate 10min
Add 100μL sample/standard, incubate 30min
Add enzyme conjugate, incubate 15min
Add substrate, incubate 15min
Add stop solution
Read at 450nm
3️⃣ Result Interpretation:
Compare to standard curve
Apply 15× dilution factor
Why Choose FSTest Malachite Green ELISA Kit?
✅ Banned Substance Focus – Specifically designed for prohibited aquaculture chemicals
✅ Unmatched Sensitivity – Lowest detection limits in the industry
✅ Matrix-Specific Protocols – Different oxidation treatments for fish vs shrimp
✅ Regulatory Ready – Complies with EU/China/USFDA zero-tolerance policies
✅ Cost-Efficient – 96 tests/kit reduces per-sample cost
Technical Specifications:
Detection Limit: 0.15ppb
LOQ: 0.01ppb
Accuracy: 70-130% recovery
Cross-Reactivity: MG (100%), LMG (20%)
Format: 96-well microplate
Storage: 2-8°C (12 months)
Regulatory Compliance:
EU Regulation (EC) No 854/2004
China GB/T 20361-2006
USFDA Import Alert 16-131
Codex Alimentarius standards