Tetracyclines ELISA Test Kit
Brand: FSTest
CatNO: DR32.5072.5
Specifications: 96tests/kit
Shelf life: 12 months
Application: Designed for quantitative detection of tetracycline-class antibiotics (including tetracycline, chlortetracycline, and oxytetracycline) in animal tissues, seafood, honey, and milk, ensuring food safety and regulatory compliance.
FSTest Tetracyclines ELISA Test Kit – High-Sensitivity Detection for Banned Antibiotic Residues
Introduction:
The FSTest Tetracyclines ELISA Test Kit is a precise immunoassay designed for quantitative detection of tetracycline-class antibiotics (including tetracycline, chlortetracycline, and oxytetracycline) in animal tissues, seafood, honey, and milk. With a 2ppb detection limit across all matrices, this kit ensures compliance with global regulations prohibiting tetracycline residues in food products.
Key Features & Benefits:
✔ Comprehensive Detection – Covers 4 key tetracyclines including: Tetracycline (100% cross-reactivity), Oxytetracycline (80%), Chlortetracycline (45%), Doxycycline (10%)
✔ Ultra-Sensitive – 2ppb detection limit meets strictest global MRLs
✔ Multi-Matrix Validation – Certified for meat, seafood, dairy, and honey
✔ Regulatory Ready – Complies with EU/China/USDA zero-tolerance policies
✔ Efficient Workflow – 96-well format processes 20+ samples per run
✔ Simplified Protocols – Matrix-specific sample preparation methods
Applications:
Livestock and poultry antibiotic monitoring
Seafood and aquaculture safety testing
Dairy product quality control
Honey export compliance verification
Veterinary drug residue surveillance
Testing Principle:
Competitive ELISA measuring absorbance at 450nm (inversely proportional to tetracycline concentration) against a 5-point standard curve (0-2.7ppb).
How to Use:
1️⃣ Sample Preparation:
Meat/Seafood: HCl extraction + pH adjustment
Honey: Simple water dilution
Milk: Trichloroacetic acid precipitation
2️⃣ Test Procedure:
Add 50μL sample/standard
Add 50μL enzyme conjugate & antibody
Incubate 30min (20-28°C)
Wash 4-5 times
Add substrate, incubate 30min
Add stop solution
Read at 450nm
3️⃣ Result Interpretation:
Compare to standard curve (0.1-2.7ppb)
Apply 10× dilution factor for all matrices
Why Choose FSTest Tetracyclines ELISA Kit?
✅ Most Regulated Antibiotic Class – Essential for compliance testing
✅ Simplified Dairy Testing – Special milk protocol with trichloroacetic acid
✅ Time-Efficient – Complete analysis in<2.5 hours
✅ Cost-Effective – 96 tests/kit reduces per-sample cost
✅ Consistent Performance – ≤10% CV ensures reliable results
Technical Specifications:
Detection Limit: 2ppb (all matrices)
LOQ: 0.2ppb
Accuracy: 70-130% recovery
Precision: ≤10% CV
Format: 96-well microplate
Storage: 2-8°C (12 months)
Regulatory Compliance:
EU Regulation 37/2010
China GB/T 21317-2007
USDA Import Monitoring
Codex Alimentarius standards