Quinolones ELISA Test Kit
Brand: FSTest
CatNO: DR32.5071.5
Specifications: 96tests/kit
Shelf life: 12 months
Application: Designed for quantitative detection of 10 major quinolone antibiotics in animal tissues, seafood, eggs, honey, and milk, ensuring food safety and regulatory compliance.
FSTest Quinolones ELISA Test Kit – Comprehensive Antibiotic Residue Detection for Food Safety
Introduction:
The FSTest Quinolones ELISA Test Kit is a high-performance immunoassay designed for quantitative detection of 10 major quinolone antibiotics in animal tissues, seafood, eggs, honey, and milk. With a 0.5ppb detection limit for most matrices (5ppb for milk), this kit provides reliable monitoring of these widely-used veterinary antibiotics to ensure food safety compliance.
Key Features & Benefits:
✔ Broad-Spectrum Detection – Covers 10 quinolones including enrofloxacin (100%), ciprofloxacin (110%), and norfloxacin (119%)
✔ Multi-Matrix Validation – Certified for 5 sample types with matrix-specific protocols
✔ Regulatory-Grade Sensitivity – 0.5ppb LOD meets global MRL requirements
✔ High-Throughput Format – 96-well design for efficient batch processing
✔ Optimized Workflow – Simplified extraction for different sample types
✔ Consistent Performance – ≤10% CV ensures reproducible results
Applications:
Livestock and poultry antibiotic monitoring
Aquaculture and seafood safety testing
Dairy and egg production quality control
Honey export compliance verification
Veterinary drug residue surveillance programs
Testing Principle:
Competitive ELISA measuring absorbance at 450nm (inversely proportional to quinolone concentration) against a 6-point standard curve (0-12ppb).
How to Use:
1️⃣ Sample Preparation:
Meat/Seafood/Eggs: Acetonitrile/dichloromethane extraction (3g sample)
Honey: Dual-phase extraction
Milk: Simple 10× dilution (100μL sample)
2️⃣ Test Procedure:
Add 50μL sample/standard
Add 50μL enzyme conjugate & antibody
Incubate 30min (20-28°C)
Wash 4-5 times
Add substrate, incubate 30min
Add stop solution
Read at 450nm
3️⃣ Result Interpretation:
Compare to standard curve (0.5-12ppb)
Apply matrix-specific dilution:
1× for tissue/seafood/eggs/honey
10× for milk
Why Choose FSTest Quinolones ELISA Kit?
✅ Most Comprehensive Coverage – Detects 10 key quinolones in one test
✅ Matrix-Specific Protocols – Optimized methods for each sample type
✅ Time-Efficient – Complete analysis in <2.5 hours
✅ Cost-Effective – 96 tests/kit reduces per-sample cost
✅ Regulatory Ready – Meets EU/China/USDA requirements
Technical Specifications:
Detection Limits:
Tissue/Seafood/Eggs/Honey: 0.5ppb
Milk: 5ppb
LOQ: 0.5ppb
Accuracy: 70-130% recovery
Precision: ≤10% CV
Format: 96-well microplate
Storage: 2-8°C (12 months)
Regulatory Compliance:
EU Maximum Residue Limits (MRLs)
China GB/T 20366-2006
USDA Food Safety Inspection
Codex Alimentarius standards