Chloramphenicol ELISA Test Kit
Brand: FSTest
CatNO: DR32.5068.5
Specifications: 96tests/kit
Shelf life: 12 months
Application: Designed for rapid and accurate detection of Chloramphenicol residues in animal tissues, seafood, honey, milk, and yogurt, ensuring food safety and regulatory compliance.
FSTest Chloramphenicol ELISA Test Kit – Ultra-Sensitive Detection for Banned Antibiotic Residues
Introduction:
The FSTest Chloramphenicol ELISA Test Kit is a high-precision immunoassay designed for quantitative detection of this prohibited antibiotic in animal tissues, seafood, honey, milk, and yogurt. With an exceptional 0.025ppb detection limit, this kit enforces compliance with global zero-tolerance regulations for chloramphenicol in food products.
Key Features & Benefits:
✔ Ultra-Sensitive Detection – 0.025ppb LOD in meat/seafood/honey, 0.1ppb in milk
✔ Regulatory Compliance – Meets EU/China/USDA zero-tolerance requirements
✔ Multi-Matrix Validation – Certified for 5 sample types including dairy
✔ High Specificity – 100% for CAP, <10% cross-reactivity (thiamphenicol)
✔ Efficient Workflow – 96-well format processes 20+ samples per run
✔ Ready-to-Use – Pre-coated plates and optimized reagents reduce setup time
Applications:
Meat/poultry safety monitoring
Seafood and honey export testing
Dairy product quality control
Veterinary drug surveillance programs
Food import/export compliance
Testing Principle:
Competitive ELISA measuring absorbance at 450nm (inversely proportional to CAP concentration) against a 6-point standard curve (0-1.2ppb).
How to Use:
1️⃣ Sample Preparation:
Meat/Seafood: Ethyl acetate extraction (3g sample)
Honey: Water/ethyl acetate dual-phase extraction
Dairy: Direct dilution (1-5mL)
2️⃣ Test Procedure:
Add 100μL sample/standard
Add 50μL enzyme conjugate & antibody
Incubate 30min (20-28°C)
Wash 4-5 times
Add substrate, incubate 30min
Add stop solution
Read at 450nm
3️⃣ Result Interpretation:
Compare to standard curve (0.05-1.2ppb)
Apply matrix-specific dilution factors:
0.5× for tissue/seafood/honey
2× for milk
5× for yogurt
Why Choose FSTest Chloramphenicol ELISA Kit?
✅ Gold-Standard Sensitivity – Lowest detection limits in industry
✅ Dairy-Specific Protocols – Unique milk/yogurt testing methods
✅ Regulatory Ready – Complies with global CAP bans
✅ Cost-Efficient – 96 tests/kit reduces per-sample cost by 40%
Technical Specifications:
Detection Limits:
Tissue/Seafood/Honey: 0.025ppb
Milk: 0.1ppb (0.05ppb with direct spotting)
Yogurt: 0.25ppb
LOQ: 0.05ppb
Accuracy: 70-130% recovery
Precision: ≤10% CV
Format: 96-well microplate
Storage: 2-8°C (12 months)
Regulatory Compliance:
EU Regulation 37/2010 (zero tolerance)
China GB/T 22338-2008
USDA Import Alert 16-04
Codex Alimentarius standards