E&O supply freshly harvested aseptically collected Donor Horse Blood and Sheep Blood. These blood products are oxygenated and supplied in both Bottles and Bags for convenient asceptical addition to culture media. Plasma products can be fractionated after the addition of a range of anticoagulants: Citrate, Alsevers, Sodium Oxolate, Heparin and EDTA.
All donor animal blood products are harvested from Stress Free Animals with guaranteed PCV’s and free from any microbiological inhibitors. The most common use is for the manufacture of Blood Agar Plates. Dehydrated culture media (Columbia Agar) is added to deionised water and then sterilized at 121°C. The molten agar is then cooled to 42°C, fresh Horse Blood or Sheep Blood is then added at a 5% or 7% concentration before being poured into petridishes.
Môi trường nuôi cấy vi sinh Rappaport-Vassilidis Broth
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Rappaport-Vassiliadis Soya Broth is used for the enrichment and selective isolation of Salmonella spp. This medium is a modification of the original formulation by Rappaport et al. and has been formulated to exploit the full characteristics of Salmonella spp. These characteristics include the ability to survive at relatively high osmotic pressure, to multiply at low pH values and greater resistance to malachite green.
This formulation also has the correct amount of magnesium chloride as previous formulations did not take into account the volume of displacement caused by dissolving large amounts of magnesium chloride in water. This formulation has been shown to be superior to tetrathionate broth and selenite broth for the isolation of Salmonella spp. from meat products.
Soya peptone provides the required carbon, nitrogen and vitamins. Potassium dihydrogen phosphate and di-potassium hydrogen phosphate act as buffers. Magnesium chloride raises the osmotic pressure in the medium. Malachite green is an inhibitory substance.
NB: This formulation is very hygroscopic and will produce a slight exothermic reaction when mixed with water.
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