E&O supply freshly harvested aseptically collected Donor Horse Blood and Sheep Blood. These blood products are oxygenated and supplied in both Bottles and Bags for convenient asceptical addition to culture media. Plasma products can be fractionated after the addition of a range of anticoagulants: Citrate, Alsevers, Sodium Oxolate, Heparin and EDTA.

All donor animal blood products are harvested from Stress Free Animals with guaranteed PCV’s and free from any microbiological inhibitors. The most common use is for the manufacture of Blood Agar Plates. Dehydrated culture media (Columbia Agar) is added to deionised water and then sterilized at 121°C. The molten agar is then cooled to 42°C, fresh Horse Blood or Sheep Blood is then added at a 5% or 7% concentration before being poured into petridishes.

Môi trường nuôi cấy vi sinh Rappaport-Vassiliadis (MSRV) Medium Semi-Solid

Rappaport-Vassiliadis (MSRV) Medium Semi-Solid is a modification of Rappaport-Vassiliadis Soy Broth for detecting motile Salmonella spp. in faeces and food products.[1]
The original research on MSRV Medium revealed a semi-solid could be used as a rapid and sensitive test for isolating motile Salmonella spp. from food products following pre-enrichment or selective enrichment.[2]

The semi-solid medium allows motility to be detected as halos of turbid growth around the original point of inoculation.
The peptones are to provide vitamins, nutrients and nitrogen to encourage growth of Salmonella spp.
The salt maintains the osmotic balance and potassium dihydrogen phosphate is a buffer for stabilising the pH of the medium.
Malachite green is included as a selective agent that inhibits Gram-positive organisms and some Gram-negative organisms.

References
(1) ISO 6579-1:2017. Microbiology of the food chain – Horizontal method for the detection, enumeration and serotyping of Salmonella – Part 1: Detection of Salmonella spp.
(2) De Smedt J.M., Bolderdikj R., Rappold H. and Lautenschlaeger D. 1986. Rapid Salmonella Detection in Foods by Motility Enrichment on a Modified Semi-Solid Rappaport-Vassiliadis Medium. J. Food Prot. 49:510-514

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