E&O supply freshly harvested aseptically collected Donor Horse Blood and Sheep Blood. These blood products are oxygenated and supplied in both Bottles and Bags for convenient asceptical addition to culture media. Plasma products can be fractionated after the addition of a range of anticoagulants: Citrate, Alsevers, Sodium Oxolate, Heparin and EDTA.
All donor animal blood products are harvested from Stress Free Animals with guaranteed PCV’s and free from any microbiological inhibitors. The most common use is for the manufacture of Blood Agar Plates. Dehydrated culture media (Columbia Agar) is added to deionised water and then sterilized at 121°C. The molten agar is then cooled to 42°C, fresh Horse Blood or Sheep Blood is then added at a 5% or 7% concentration before being poured into petridishes.
Môi trường nuôi cấy vi sinh Mannitol Salt Agar
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Mannitol Salt Agar is selective medium for the isolation of pathogenic staphylococci. The medium conforms to the requirements of the Harmonised USP/EP/JP. Chapman showed that adding a high level of sodium chloride to Phenol Red Mannitol Agar allowed for the recovery of pathogenic staphylococci. Pathogenic staphylococci produced yellow colonies whereas non-pathogenic staphylococci produced small red colonies.
Pancreatic digest of casein, peptic digest of animal tissue and beef extract provide the required nitrogen, carbon and vitamins. The high level of sodium chloride inhibits most organisms other than staphylococci. Mannitol is a carbohydrate that is fermented by coagulase positive staphylococci. Phenol red is the pH indicator.
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