E&O supply freshly harvested aseptically collected Donor Horse Blood and Sheep Blood. These blood products are oxygenated and supplied in both Bottles and Bags for convenient asceptical addition to culture media. Plasma products can be fractionated after the addition of a range of anticoagulants: Citrate, Alsevers, Sodium Oxolate, Heparin and EDTA.
All donor animal blood products are harvested from Stress Free Animals with guaranteed PCV’s and free from any microbiological inhibitors. The most common use is for the manufacture of Blood Agar Plates. Dehydrated culture media (Columbia Agar) is added to deionised water and then sterilized at 121°C. The molten agar is then cooled to 42°C, fresh Horse Blood or Sheep Blood is then added at a 5% or 7% concentration before being poured into petridishes.
Môi trường nuôi cấy vi sinh MRS Agar (de Man, Rogosa & Sharpe )
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This medium is used for the cultivation and enumeration of Lactobacillus spp. MRS agar is based on the formulation by de Man, Rogosa and Sharpe.
The peptone, yeast extract and beef extract provides the reuired carbon, nitrogen and vitamin source. Glucose is a fermentable carbohydrate. The magnesium sulphate and manganese sulphate act as growth stimulants. Potassium phosphate is a buffering agent. Selectivity of the medium is achieved through the use of ammonium citrate and sodium acetate, inhibiting microorganisms such as streptococci and moulds. The addition of the surfactant Tween 80 is required to facilitate the uptake of nutrients from Lactobacillus spp.
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