It is not possible to sterilise whole blood products and therefore they must be collected aseptically. Horse and sheep blood are the most widely used animal blood products in culture media. The choice of which type of blood to use with culture media is largely traditional, with much of continental Europe preferring sheep blood, whilst the UK and certain parts of the Commonwealth prefer horse blood. Defibrinated horse blood is aseptically collected whole horse blood that has been processed to remove fibrin. There are no additives or preservatives in this product. Defibrination is now accepted as the best method of preventing blood clotting. It must be carried out immediately after drawing the blood and the agitation must be sufficient to denature the fibrinogen but not to cause rupture of the erythrocytes and haemolysis. The haemolytic reactions of horse blood are not identical to sheep blood and blood agar media designed for horse blood may not be satisfactory with sheep blood and vice versa.
Huyết thanh ngựa vô trùng là chất bổ sung cần thiết cho điều chế môi trường nuôi cấy vi sinh,
- Intended Use
Sterile Horse Serum (SHS) is an aseptically collected reagent, for supplementation of culture media, derived from defibrinated horse blood. - Composition
Ingredients
Filtered serum from antibiotic free 100% defibrinated Horse Blood
*Adjusted/supplemented as needed to meet performance requirements - Summary, Explanation and Principles
Sterile Horse Serum is suitable for use, as a supplement in the preparation of culture media. Sterile horse serum can be used as a nutrient additive to both broths and agars in microbiological testing. Sterile Horse Serum is collected from antibiotic free herds located in the UK. The serum is processed through a triple filter filtration system to 0.2µm. After filtration the dispensing and bottle filling processes are carried out in a clean room area under laminar flow. Once labelled, the filled bottles are frozen and stored at -20°C without delay. - Preparation Instructions
Dependent on final medium being produced, aseptically add the required amount of Sterile Horse Serum (SHS) to the cooled medium and allow to mix prior to final product dispensing.
It is not possible to sterilise whole blood products and therefore they must be collected aseptically. Horse and sheep blood are the most widely used animal blood products in culture media. The choice of which type of blood to use with culture media is largely traditional, with much of continental Europe preferring sheep blood, whilst the UK and certain parts of the Commonwealth prefer horse blood. Defibrinated sheep cells are aseptically collected whole sheep blood that has been processed to remove fibrin. There are no additives or preservatives in this product. Defibrination is now accepted as the best method of preventing blood clotting. It must be carried out immediately after drawing the blood and the agitation must be sufficient to denature the fibrinogen but not to cause rupture of the erythrocytes and haemolysis. The haemolytic reactions of sheep blood are not identical to the reactions of horse blood and blood agar media designed for sheep blood may not be satisfactory with horse blood and vice versa.
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All E&O products are for in vitro use only. E&O products are intended only for use by qualified professionals who will safely handle and dispose of products they receive. All biologically derived materials (e.g. blood, sera) should be handled as if a potential biohazard. E&O media that contain antibiotics should be handled with care. Chemical resistant gloves, eye protection and laboratory coat should be worn.
E&O Heat Inactivated Serum is derived from horse serum and is suitable for use in diagnostic assays.
One of the reasons for the heat inactivation of serum (heating to 56°C for 30 min) is to inactivate complement, a group of proteins present in serum that are part of the immune response.
This is sometimes important for cells that will be used to prepare or assay viruses, used in cytotoxicity assays or other systems where complement may have an unwanted influence.
The use of Heat Inactivated Serum is also usually recommended for growing embryonic stem cells.
After filtration the dispensing and bottle filling processes are carried out in a state-of-the-art clean room under laminar flow.
Once labelled the filled bottles are then subjected to controlled heat inactivation and are frozen and stored at -20°C without delay.
The filter sterile Heat Inactivated Horse Serum is supplied in 100 or 500 ml PETG bottles.
All E&O products are for in vitro use only.
E&O products are intended only for use by qualified professionals who will safely handle and dispose of products they receive.
All biologically derived materials (e.g. blood, sera) should be handled as if a potential biohazard.
E&O media that contain antibiotics should be handled with care.
Chemical resistant gloves, eye protection and laboratory coat should be worn.
Lysed horse blood is used for special purposes in culture media. It has been used for many years in Corynebacterium diphtheriae media, where better growth was observed after lysis of the horse blood by the tellurite in the medium. It is also documented that lysed blood stimulates the growth of Haemophilus influenzae due to the release of Nicotinamide Adenine Dinucleotide in the horse blood from the ruptured erythrocytes. In antibiotic susceptibility testing, lysed horse blood is added to the medium to improve the reactions with trimethoprim and sulphonamides. Most culture media, unless specially processed for susceptibility testing, contain amounts of thymidine which can antagonise the inhibitory effects of these antimicrobials. When horse blood is lysed the erythrocytes release an enzyme thymidine phosphorylase which converts thymidine into the much less antagonistic compound thymine.
It is not possible to sterilise whole blood products and therefore they must be collected aseptically. Horse and sheep blood are the most widely used animal blood products in culture media. The choice of which type of blood to use with culture media is largely traditional, with much of continental Europe preferring sheep blood, whilst the UK and certain parts of the Commonwealth prefer horse blood. Defibrinated sheep cells are aseptically collected whole sheep blood that has been processed to remove fibrin. There are no additives or preservatives in this product. Defibrination is now accepted as the best method of preventing blood clotting. It must be carried out immediately after drawing the blood and the agitation must be sufficient to denature the fibrinogen but not to cause rupture of the erythrocytes and haemolysis. The haemolytic reactions of sheep blood are not identical to the reactions of horse blood and blood agar media designed for sheep blood may not be satisfactory with horse blood and vice versa.
It is not possible to sterilise whole blood products and therefore they must be collected aseptically. Horse and sheep blood are the most widely used animal blood products in culture media. The choice of which type of blood to use with culture media is largely traditional, with much of continental Europe preferring sheep blood, whilst the UK and certain parts of the Commonwealth prefer horse blood. Defibrinated horse blood is aseptically collected whole horse blood that has been processed to remove fibrin. There are no additives or preservatives in this product. Defibrination is now accepted as the best method of preventing blood clotting. It must be carried out immediately after drawing the blood and the agitation must be sufficient to denature the fibrinogen but not to cause rupture of the erythrocytes and haemolysis. The haemolytic reactions of horse blood are not identical to sheep blood and blood agar media designed for horse blood may not be satisfactory with sheep blood and vice versa.