Môi trường vi sinh EOLabs

E&O Laboratories Ltd have successfully grown their Culture Media business over the last 30 years to become a leading manufacturer
servicing Microbiology Laboratories , Location in Scotland. Their comprehensive media range is continually expanding into specialised industry sectors as well as aiding the rapid diagnosis of the newest antibiotic resistant bacteria strains.

INDUSTRY SECTORS:
• Clinical, Veterinary & Aquaculture
• Food, Water & Environmental
• Pharmaceutical, Industrial & Cosmetics
• Academia & Research

PRODUCT CATEGORIES:
• Ready-to-use Culture Media – Bottled, Bagged and Plated
• Dehydrated Culture Media & Raw Materials
• Antibiotic Supplements & Reagents
• Fresh Donor Horse & Sheep Blood – Frozen Sterile Filtered Serum

Dehydrated Culture Media products are formulated to supply the required nutrients to allow for the growth of microorganisms. Used in combination with a variety of selective agents and incubation conditions a wide range of specific organisms can be isolated. With careful raw material selection of the various media components E&O can ensure a consistent level of quality and performance. For each formulation the necessary ingredients are accurately weighed, combined and blended together to produce a homogenous powdered product.

Môi trường vi sinh Alphachem

Môi trường nuôi cấy vi sinh AgarCult Lysine Decarboxylase Broth IS

Of Glucose Agar

Glucose Agar

Drigalski Lactose Agar

Bromocresol Purple Glucose Agar

Môi trường vi sinh EE Broth (Mossel)

Môi trường nuôi cấy vi sinh Kligler Iron Agar

Môi trường vi sinh TSI AGAR (ISO 6579-1)

Môi trường vi sinh Alphachem

Môi trường nuôi cấy vi sinh AgarCult Lysine Decarboxylase Broth IS

Principles and uses
Lysine Decarboxylase Broth is used to detect and differentiate Enterobacteria from other microorganisms, based on lysine decarboxylation.
Gelatin peptone provides nitrogen, vitamins, minerals and amino acids essential for growth.Yeast extract is a source of vitamins, particularly of the B-group. Dextrose is the fermentable carbohydrate. Bromocresol purple is the pH indicator. Lysine is added to detect the production of the specific enzyme.
When the medium is inoculated with a bacterium that is able to ferment dextrose, the acid produced lowers the pH of the medium and changes the color of the indicator from purple to yellow. The acidic condition also stimulates decarboxylase activity. The bacteria that decarboxylate the L-Lysine to cadaverine are identified by the presence of a purple-red color. The production of these amines elevates the pH of the medium. A yellow color after 24 hours indicates a negative result. By substituting L-Lysine with Arginine or Ornithine, the new resulting medium (Falkow Broth Base) can be used to study the decarboxylation of these amino acids.

Preparation
Suspend 14 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense quantities of 5 ml into screw-capped tubes. Sterilize in autoclave at 121 ºC for 15 minutes. Leave caps loose to allow gas exchange. Close well after sterilization.

Instructions for use

Gently insert the inoculation loop with the sample into the tube and move the loop back and forth several times to inoculate the media.
Incubate at 35±2 °C for 24 hours
Color change to purple: positive decarboxylation reaction (Escherichia, Klebsiella, Salmonella, except S.parathyphi, Arizona, Alkalescens Dispar, Serratia).
Yellow color: negative decarboxylation reaction (Proteus, Providencia, S.parathypi A,Shigella, Aeromonas, Citrobacter).

Of Glucose Agar

The OF Glucose agar allows the demonstration of glucose fermentation as an identification test for Enterobacteriaceae in the context of the following standards : NF EN ISO 21528-1 and NF EN ISO 21528-2 for the detection and enumeration of Enterobacteriaceae.

Glucose Agar

Glucose agar allows the demonstration of glucose fermentation (with or without gas production) as an identification test for Enterobacteriaceae or Pseudomonas spp..

Drigalski Lactose Agar

Drigalski Lactose agar is used for the selective isolation of Enterobacteriaceae. It allows the differentiation of bacteria according to their ability to ferment lactose in food, pharmaceutical, cosmetic and veterinary samples.

Bromocresol Purple Glucose Agar

Bromocresol Purple Glucose Agar for the detection and enumeration of Enterobacteriaceae by ISO 7402, ISO 8523, ISO 21528

Principles and uses
Bromocresol Purple Glucose Agarr is used for the differentiation of Enterobacteriaceae in urine, water and food. It differentiates species on the basis of dextrose fermentation.
This medium is recommended by ISO 11059 for the confirmation of Pseudomonas spp. in milk and milk products. Bromocresol Purple Glucose Agar was also recommended by ISO 21528:2014 for the confirmation of Enterobacteriaceae.
Tryptone and Yeast extract provide nitrogen, vitamins, minerals and amino acids essential for growth. D-glucose is the fermentable carbohydrate providing carbon and energy Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bromocresol purple is a pH indicator. Bacteriological agar is the solidifying agent.
The glucose-fermenting microorganisms produce yellow colonies (acid) and the non-fermenting ones, purple colonies. Colonies that are oxidasenegative and glucose-positive are confirmed as Enterobacteriaceae.

Preparation

Suspend 41.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes.

Môi trường vi sinh EE Broth (Mossel)

E.E. Broth is recommended as an enrichment medium when examining food and feedstuffs for Enterobacteriaceae.
It is a modification of Brilliant Green Bile Broth, with an improved buffering capacity to encourage early growth and prevent autosterilization.

E.E. Broth uses glucose instead of lactose to make the medium a test for all enterobacteria including non-lactose fermenting organisms.
This formulation complies with the Harmonized USP/EP/JP.

Nitrogen is supplied by the gelatin peptone whilst glucose serves as the fermentable carbohydrate source.
Oxbile and brilliant green are the selective agents helping to suppress Gram-positive non- target organisms.
Auto sterilisation is prevented through the buffer system composed of potassium dihydrogen phosphate and disodium hydrogen phosphate.

Để được tư vấn chi tiết, giải đáp thắc mắc, hướng dẫn sử dụng và báo giá cụ thể, hãy liên hệ ngay với Labcare VN – Công ty TNHH Labcare Việt Nam qua các thông tin bên dưới:

Email: info@labcare.vn
Hotline: 0938976508
Website: http://labcare.vn | http://moitruongvisinh.vn

Môi trường nuôi cấy vi sinh Kligler Iron Agar

Kligler iron agar is used to differentiate between some of the enterobacteriacae on the basis of three reactions: fermentation of lactose and glucose and the production of hydrogen sulphide.
Kligler iron agar is a modification of the original formulation developed by Kligler.
It incorporates the principles of Russell’s double sugar agar with Kligler ‘s lead acetate agar.
The peptone provides the required nitrogen, carbon and vitamins. Lactose and glucose are carbohydrates.
Acid production from their fermentation is detected by the phenol red pH indictor. Sodium thiosulphate is reduced to hydrogen sulphide which is detected by the ferric citrate indicator.
Sodium chloride maintains the osmotic balance.

Liên hệ mua hàng:

Công ty Labcare Việt Nam – chuyên cung cấp, phân phối Môi trường nuôi cấy vi sinh, test sinh hóa, kháng huyết thanh, hóa chất, dụng cụ, thiết bị, vật tư tiêu hao.

Hotline: 0938976508

Email: info@labcare.vn

Website: labcare.vn

Môi trường vi sinh TSI AGAR (ISO 6579-1)

TSI (Triple Sugar Iron) Agar is used for the identification of enterobacteria by the rapid detection of the fermentation of lactose, glucose (with or without gas production) and of sucrose, as well as the production of hydrogen sulfide.
The typical composition corresponds to that defined in the standards NF EN ISO 6579-1 and NF EN ISO 19250 for the detection of Salmonella spp..

Môi trường vi sinh Alphachem

Môi trường nuôi cấy vi sinh AgarCult Lysine Decarboxylase Broth IS

Principles and usesLysine Decarboxylase Broth is used to detect and differentiate Enterobacteria from other microorganisms, based on lysine decarboxylation.Gelatin peptone provides nitrogen, vitamins, minerals and amino acids essential for growth.Yeast extract is a source of vitamins, particularly of the B-group. Dextrose is the fermentable carbohydrate. Bromocresol purple is the pH indicator. Lysine is added to detect the production of the specific enzyme.When the medium is inoculated with a bacterium that is able to ferment dextrose, the acid produced lowers the pH of the medium and changes the color of the indicator from purple to yellow. The acidic condition also stimulates decarboxylase activity. The bacteria that decarboxylate the L-Lysine to cadaverine are identified by the presence of a purple-red color. The production of these amines elevates the pH of the medium. A yellow color after 24 hours indicates a negative result. By substituting L-Lysine with Arginine or Ornithine, the new resulting medium (Falkow Broth Base) can be used to study the decarboxylation of these amino acids. PreparationSuspend 14 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense quantities of 5 ml into screw-capped tubes. Sterilize in autoclave at 121 ºC for 15 minutes. Leave caps loose to allow gas exchange. Close well after sterilization. Instructions for use Gently insert the inoculation loop with the sample into the tube and move the loop back and forth several times to inoculate the media. Incubate at 35±2 °C for 24 hours Color change to purple: positive decarboxylation reaction (Escherichia, Klebsiella, Salmonella, except S.parathyphi, Arizona, Alkalescens Dispar, Serratia). Yellow color: negative decarboxylation reaction (Proteus, Providencia, S.parathypi A,Shigella, Aeromonas, Citrobacter). Read More

Of Glucose Agar

Glucose Agar

Glucose agar allows the demonstration of glucose fermentation (with or without gas production) as an identification test for Enterobacteriaceae or Pseudomonas spp.. Read More

Drigalski Lactose Agar

Bromocresol Purple Glucose Agar

Bromocresol Purple Glucose Agar for the detection and enumeration of Enterobacteriaceae by ISO 7402, ISO 8523, ISO 21528 Principles and usesBromocresol Purple Glucose Agarr is used for the differentiation of Enterobacteriaceae in urine, water and food. It differentiates species on the basis of dextrose fermentation.This medium is recommended by ISO 11059 for the confirmation of Pseudomonas spp. in milk and milk products. Bromocresol Purple Glucose Agar was also recommended by ISO 21528:2014 for the confirmation of Enterobacteriaceae.Tryptone and Yeast extract provide nitrogen, vitamins, minerals and amino acids essential for growth. D-glucose is the fermentable carbohydrate providing carbon and energy Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bromocresol purple is a pH indicator. Bacteriological agar is the solidifying agent.The glucose-fermenting microorganisms produce yellow colonies (acid) and the non-fermenting ones, purple colonies. Colonies that are oxidasenegative and glucose-positive are confirmed as Enterobacteriaceae. Preparation Suspend 41.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes. Read More

Môi trường vi sinh EE Broth (Mossel)

E.E. Broth is recommended as an enrichment medium when examining food and feedstuffs for Enterobacteriaceae.It is a modification of Brilliant Green Bile Broth, with an improved buffering capacity to encourage early growth and prevent autosterilization. E.E. Broth uses glucose instead of lactose to make the medium a test for all enterobacteria including non-lactose fermenting organisms.This formulation complies with the Harmonized USP/EP/JP. Nitrogen is supplied by the gelatin peptone whilst glucose serves as the fermentable carbohydrate source.Oxbile and brilliant green are the selective agents helping to suppress Gram-positive non- target organisms.Auto sterilisation is prevented through the buffer system composed of potassium dihydrogen phosphate and disodium hydrogen phosphate. Để được tư vấn chi tiết, giải đáp thắc mắc, hướng dẫn sử dụng và báo giá cụ thể, hãy liên hệ ngay với Labcare VN – Công ty TNHH Labcare Việt Nam qua các thông tin bên dưới: Email: info@labcare.vn Hotline: 0938976508 Website: http://labcare.vn | http://moitruongvisinh.vn Read More

Môi trường nuôi cấy vi sinh Kligler Iron Agar

Kligler iron agar is used to differentiate between some of the enterobacteriacae on the basis of three reactions: fermentation of lactose and glucose and the production of hydrogen sulphide.Kligler iron agar is a modification of the original formulation developed by Kligler.It incorporates the principles of Russell’s double sugar agar with Kligler ‘s lead acetate agar.The peptone provides the required nitrogen, carbon and vitamins. Lactose and glucose are carbohydrates.Acid production from their fermentation is detected by the phenol red pH indictor. Sodium thiosulphate is reduced to hydrogen sulphide which is detected by the ferric citrate indicator.Sodium chloride maintains the osmotic balance. Liên hệ mua hàng: Công ty Labcare Việt Nam – chuyên cung cấp, phân phối Môi trường nuôi cấy vi sinh, test sinh hóa, kháng huyết thanh, hóa chất, dụng cụ, thiết bị, vật tư tiêu hao. Hotline: 0938976508 Email: info@labcare.vn Website: labcare.vn Read More

Môi trường vi sinh TSI AGAR (ISO 6579-1)

TSI (Triple Sugar Iron) Agar is used for the identification of enterobacteria by the rapid detection of the fermentation of lactose, glucose (with or without gas production) and of sucrose, as well as the production of hydrogen sulfide.The typical composition corresponds to that defined in the standards NF EN ISO 6579-1 and NF EN ISO 19250 for the detection of Salmonella spp.. Read More