E&O Laboratories Ltd have successfully grown their Culture Media business over the last 30 years to become a leading manufacturer
servicing Microbiology Laboratories , Location in Scotland. Their comprehensive media range is continually expanding into specialised industry sectors as well as aiding the rapid diagnosis of the newest antibiotic resistant bacteria strains.

INDUSTRY SECTORS:
• Clinical, Veterinary & Aquaculture
• Food, Water & Environmental
• Pharmaceutical, Industrial & Cosmetics
• Academia & Research

PRODUCT CATEGORIES:
• Ready-to-use Culture Media – Bottled, Bagged and Plated
• Dehydrated Culture Media & Raw Materials
• Antibiotic Supplements & Reagents
• Fresh Donor Horse & Sheep Blood – Frozen Sterile Filtered Serum

Dehydrated Culture Media products are formulated to supply the required nutrients to allow for the growth of microorganisms. Used in combination with a variety of selective agents and incubation conditions a wide range of specific organisms can be isolated. With careful raw material selection of the various media components E&O can ensure a consistent level of quality and performance. For each formulation the necessary ingredients are accurately weighed, combined and blended together to produce a homogenous powdered product.

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Bromocresol Purple Glucose Agar-image

Bromocresol Purple Glucose Agar

  • Product Details
  • APPLICATIONS
  • SPECIFICATIONS
  • CODE
  • DOCUMENTS

Bromocresol Purple Glucose Agar for the detection and enumeration of Enterobacteriaceae by ISO 7402, ISO 8523, ISO 21528

Principles and uses
Bromocresol Purple Glucose Agarr is used for the differentiation of Enterobacteriaceae in urine, water and food. It differentiates species on the basis of dextrose fermentation.
This medium is recommended by ISO 11059 for the confirmation of Pseudomonas spp. in milk and milk products. Bromocresol Purple Glucose Agar was also recommended by ISO 21528:2014 for the confirmation of Enterobacteriaceae.
Tryptone and Yeast extract provide nitrogen, vitamins, minerals and amino acids essential for growth. D-glucose is the fermentable carbohydrate providing carbon and energy Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bromocresol purple is a pH indicator. Bacteriological agar is the solidifying agent.
The glucose-fermenting microorganisms produce yellow colonies (acid) and the non-fermenting ones, purple colonies. Colonies that are oxidasenegative and glucose-positive are confirmed as Enterobacteriaceae.

Preparation

Suspend 41.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes.

For the confirmation of Enterobacteriaceae and Pseudomonas.
Bacteriological agar 15 Bromocresol purple 0.015 D-Glucose 10 Sodium chloride 5 Tryptone 10 Yeast extract 1.5
BPGA500
TDS
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