Egg Yolk Agar Base is used for isolation and identification of Clostridia and certain other anaerobes. This medium is used for isolation and detection of Clostridium perfringens and differs from the original formula by the inclusion of hemin.
This media is prepared in accordance with the microbial limit testing by harmonized methodology of USP/EP/BP/JP/IP. It is recommended for sterility checking of non-sterile products, nutritional and dietary supplements. It can be used to initiate growth from small inocula and to obtain the highest viable count of clostridia. Barnes and Ingram used the broth medium for diluting an inoculum of vegetative cells of Clostridium perfringens. It can be used in studies of spore forming anaerobes. Composition complies with specifications in Chapter 62 Microbiological Examination of Nonsterile Products: Tests for Specified microorganism.
Cooked Meat Medium (R.C.Medium) is used for cultivation of aerobes and anaerobes, especially pathogenic Clostridia and also for the maintenance of stock cultures.
Campylobacter Cefex Agar Base is recommended for isolation and cultivation of Campylobacter species. It is a highly nutritious base and the addition of horse blood supplements the medium with X-factor and other growth factor requirements.Incorporation of antibiotics, Cefaperazone and cycloheximide inhibits gram positiv eand gram negative organisms and fungi.Typical Campylobacter jejuni appear as small mucoid, grayish flat colonies with irregular edges.
Karmali Campylobacter Agar Base is used for selective isolation and cultivation of thermotolerant Campylobacter species from food and animal feeds as per APHA.This medium is recommended by the ISO Committee and APHA for the selective isolation and cultivation of Campylobacter species and is a modification of the original formulation of Karmali et al.
Modified Rappaport Vassiliadis Medium is recommended for selective enrichment of Salmonellae from food and environmental specimens. This medium is a selective enrichment medium for isolation of Salmonella from foodstuffs, environmental and clinical specimens. The test specimen is to be first added to Buffered Peptone Water for pre-enrichment followed by inoculation into Modified Rappaport Vassiliadis Medium and and further subculturing on Brilliant Green Agar.
Yersinia enterocolitica Selective enrichment
RPF Agar Base is recommended for the enumeration of coagulase positive Staphylococci from food and animal feeding stuffs. The composition and performance criteria are in accordance with ISO 6888-2:1999 & / Amd 1:2003. This medium works on same principle as Baird Parker Agar with addition of Fibrinogen Plasma together with Trypsin and Potassium Tellurite as single supplement. The medium is used for isolation and enumeration of Staphylococci in food and other material since it allows a good differentiation of coagulase positive strains.Staphylococci appera as white to grey-black coloured colony surrounded by an opaque zone.
Ringer Salt Solution, Granulated is recommended an isotonic diluent for food, milk and dairy products during microbiological examination. It is physiologically superior to physiological saline for sensitive organisms andused for dilutions =during microbiological examination protocol.
This medium was developed by Lowburry and is a modification of Tech Agar (Medium A) with addition of cetrimide for selective isolation of P. Aeruginosa. These media are used for the examination of cosmetics and clinical specimens for the presence of Ps.aeruginosa, as well as for evaluating the efficacy of disinfectants against this organism. Ps.aeruginosa colonies may appear pigmented blue, blue-green or non-pigmented. Colonies exhibiting fluorescence at 250nm and a blue green pigmentation are considered as presumptive positive.
Cetrimide Broth is recommended for selective cultivation of Pseudomonas aeruginosa. This medium is the modification of theformula designed by King, Ward and Raney and is useful for the cultivation of P. aeruginosa. This medium is selective as it contains cetrimide (Cetyl trimethyl ammonium bromide), which inhibits other bacteria except P.aeruginosa. is used for the examination of cosmetics and clinical specimens for the presence of P. aeruginosa, as well as for evaluating the efficacy of disinfectants against this organism.
Recommended as a diluent for carrying out microbial limit testingby harmonized methodology of pharmaceutical products in accordance with USP/EP/BP/JP/IP, Composition complies with specifications in Chapter 62 Microbiological Examination of Nonsterile Products: Tests for Specified microorganism
For the isolation and identification of Cronobacter sakazakii from food products.The composition and performance of this media are as per specifications laid down in in ISO /TS 22964: 2017.
Recommended for the isolation and identification of Cronobacter sakazakii from food, dairy and clinical products.
Sporulation Broth is recommended for promoting sporulation in Bacillus subtilis. It is used for the detection of antibiotic residues in milk and milk products. The medium is specified for the preparation of spores, by APHA, for disc assay procedure for the detection of sulfa drugs and antibiotics in milk.
Lactose Gelatin Medium is recommended for detection of Clostridium species from food samples. The medium contains lactose which is fermented by the Clostridium species yielding acid and gas. Phenol red acts as the pH indicator which turns yellow at acidic pH. Clostridium perfringens shows positive lactose fermentation and gelatin liquefaction.
Iron Medium Base is recommended for presumptive confirmation of Clostridium perfringens from from food in accordance with FDA BAM, 1998.iOn isolation of black colonies from suspected foods on TSC agar, the culture is enriched in Fluid thioglycollate medium. The enriched culture is tested for stormy fermentation in this medium with added whole milk ,
CL Broth (Chopped Liver Broth) is recommended for the cultivation and enrichment of anaerobic bacteria from food It is in accordance with APHA and also recommended by FDA . HL Peptone B solids and peptone provide nitrogen and other nutrients necessary to support bacterial growth. Dipotassium phosphate provides buffering to the medium. Starch serves as a source of carbon.
Perfringens Agar Base, Granulated (Tryptose Sulphite Cycloserine Agar Base, Granulated) (T.S.C./S.F.P. Agar Base, Granulated) is recommended for the presumptive identification and enumeration of Clostridium perfringens with the addition of selective supplement and enrichment.This medium has been documented as one of the most useful media for the quantitative recovery of C.perfringens while suppressing growth of other facultative anaerobes, also recommended by APHA.
Medium for the differentiation of urea-metabolizing microorganisms, according to ISO 6579, ISO 10273, ISO 19250, ISO 21567.
Recommended for identification of Yersinia enterocolitica from dairy products
V Infusion Agar (Formerly known as Veal Infusion Agar) is recommended for the cultivation of fastidious pathogenic bacteria. The medium is used in preparation of stock cultures of Escherichia coli, in preparation of E. coli cultures to test their ability in invading mammalian cells and in microbial examination of egg and egg products.
Shigella Broth Base is recommended for the isolation and cultivation of Shigella species from food. It is recommended by APHA.
ISP Medium No. 7 (Tyrosine Agar) medium is recommended for the isolation and enumeration of Streptomyces species. It is used for the differentiation of Streptomyces species based on tyrosine utilization. Zone of clearance around the colony indicates tyrosine hydrolysis.
recommended to determine the fermentation reaction of carbohydrates for differentiation of microorganisms. The medium serves as a differential medium for differentiation of various species and genera, by the production of acid or acid and gas.
CPC Agar Base w/ 1% cellobiose is used for cultivation and identification of Vibrio species from food in accordance with FDA BAM, 1998. It designed to differentiate Vibrio vulnificus from other Vibrios. Vibrio cholerae strains except V. cholerae 01-classical biotype grow on CPC Agar while most Vibrio parahaemolyticus strains do not grow on CPC Agar. Cellobiose is fermented by some Vibrio’s producing acid and is indicated by the pH indicator bromothymol blue, which turns yellow at acidic pH. Cresol red is the pH indicator of alkaline range, which turns red at alkaline pH. Alkaline pH of the medium enhances the recovery of Vibrio’s.
Casitose Yeast Extract Broth (CAYE) is recommended for cultivation of Vibrio cholerae cultures while testing their enterotoxigenicity. This medium is formulated as per APHA for cultivating Vibrio cholerae while testing their enterotoxigenicity as these media enhance the production of Vibrio enterotoxin. The medium contents like casein acid hydrolysate and yeast extract provide the essential nitrogenous nutrients and B-vitamins to the growing Vibrio’s.
Diferential solid medium used for the presuntive identification of Vibrio ssp
AKI Medium is used for identification of Vibrio in accordance with FDA BAM, 1998.It is used for the serological identification of Cholera enterotoxin of Vibrio species .
Blood Free Campylobacter Selectivity Agar Base w/ Yeast Extract is recommended for selective isolation and differentiation of Campylobacter species, in accordance with FDA BAM, 1998. According to the BAM protocol, samples are pre enriched if required with suitable media under micro aerobic conditions for appropriate time at 37°C. These enriched cultures are further proceeded for isolation, identification and confirmation.
Campylobacter Enrichment Broth Base (Preston Enrichment Broth Base) is recommended for selective enrichment and cultivation of Campylobacter species. This medium is recommended by APHA for enrichment of thermotolerant Campylobacter species from foods. It has a rich basal medium to aid resuscitation of sublethally damaged Campylobacter. Preliminary incubation of the medium complete with antibiotics for 4 hours at 37°C was recommended to aid resuscitation of injured organisms followed by 42°C for 18-48 hours.
M-Endo Agar LES is recommended for enumeration of coliforms in water using a two step membrane filtration technique. It is used for testing coliforms in water using a two-step membrane filter procedure, wherein Lauryl Sulphate Broth (M080) is used as the primary enrichment medium. This medium is recommended by APHA for testing coliforms in drinking and in bottled water. Presumptive coliform bacteria will form red colonies with metallic sheen after an incubation at 35-37°C for 24 hours.
M-Endo Broth MF (MF Endo Medium) (M-Coliform Broth) is recommended for enumeration of coliform bacteria in water samples using one step membrane filtration technique by differentiating between lactose-fermenters and non-fermenters. This medium employed sodium sulphite and basic fuchsin instead of bile salts to achieve inhibition of gram-positive bacteria.
Malt Agar is recommended by APHA for use in both antibiotic and acidified standard methods for yeast and mould counts in food. This medium is also used for maintaining stock cultures of fungi. With acidic pH, they are used for the isolation, cultivation and maintenance of yeast and moulds.
Buffered Listeria Enrichment Broth Base is recommended by FDA Committee for enrichment in isolation of Listeria
monocytogenes
Motility Test Medium is recommended for detection of bacterial motility. Culture is stab inoculated through the centre of the medium and iIncubated at appropriate temperature. Non-motile organisms grow only along the line of inoculation whereas motile organisms grow away from the line of inoculation or may show growth even throughout the medium.
used as a base for studying citrate utilization tests. This medium is recommended by APHA, and others, to presumptively identify coliforms encountered in the food and dairy industry
Malonate Broth is recommended for differentiation of Enterobacter and Escherichia on the basis of malonate utilization. Malonate utilizers change the colour of the medium to blue due to bromothymol blue, pH indicator in the medium. Enterobacter aerogenes, Klebsiella pneumoniae, Salmonella Arizonae are known malonate utilizers, the colour of medium changes to dark blue due to malonate utilization. Escherichia coli and Salmonella Typhimurium are malonate negative.
Mucate Broth is used for identification of enteropathogenic Escherichia coli and Salmonella species from milk and milk products as per APHA. This medium is formulated by Kauffman and Peterson and is recommended by APHA for identification of enteropathogenic Escherichia coli from milk and milk products. This medium can also be used as an aid in differentiation of Enterobacteriaceae especially within Salmonella genus.
Acetate Agar is used for the isolation and cultivation of Leuconostoc and Pediococcus species. This medium was formulated by Whittenbury and then modified by Keddie for the isolation and cultivation of Leuconostoc and Pediococcus species.
for identification of microorganisms by means of nitrate reduction and indole Production.
This medium is recommended for performance of Methyl Red and Voges Proskauer tests in differentiation of coli-aerogenes group. It forms a part of IMViC test in the biochemical panel. It is available in 5 ml quantity.
For differentiation of the members of Enterobacteriaceae on the basis of potassium cyanide tolerance.
for differentiating Salmonella arizonae from the Bethesda Bellerup group of
Enterobacteriaceae.
For the detection of indole production by coliforms from clinical and non-clinical samples.
Tryptone Phosphate Broth is recommended for enrichment and cultivation of enteropathogenic Escherichia coli from
suspected food samples
Recommended for selective detection and enumeration of Campylobacter species from food chain as per the specifications laid down in ISO 10272-2:2017.
Bacteriological peptone is an economical source of nutrients provided by a balanced mixture of meat peptones and tryptone.The growth requirements of most non fastidious organisms will be fulfilled by the range of amino acids, peptides and proteoses in this mixture.
A selective broth for the growth of coliforms and Escherichia coli from food and environmental samples.
For the detection of Escherichia coli in water and food samples by a fluorogenic method.
M-Staphylococcus Broth is used for detection and isolation of Staphylococci by membrane filter technique.
Recommended for selective isolation and identification of pathogenic Staphylococcus aureus by detecting lipase production and mannitol fermentation from clinical and non-clinical specimens as per APHA.
Solid differential medium used for the selective isolation of Yersinia spp. from highly polluted samples,
according to ISO 10273 standard.
This medium requires add Yersinia Selective Supplement (Art. No. 06-143LYO1).
olid medium for the detection and isolation of Alicyclobacillus, in fruit juices and other acidic food, according
to IFU standard method No. 12.
- After autoclaving adjust the pH to 3, 7 ±0.2 by adding 1N HCl.
Liquid medium for the culture and enumeration of yeast and bacteria for microbiological control in brewing and
other fermentation industries by MF method.
Base medium, to which beer may be added for the cultivation of microbial contaminants in the brewing industry
Liquid medium for the selective enrichment of Cronobacter sakazakii in milk samples and diary products,
according to the ISO 22964. Needs Vancomycin Selective Supplement (Art. No. 06-712-LYO1). The prepared
media (Art. No. 064-TA 0196) include Vancomycin.
Liquid medium used for the production of indol and gas in a single tube, according to ISO 9308-1 and 9308-2
standards.
Solid medium for the enumeration of water microorganisms according to ISO 6222, 5667-2, 5667-3, 6887
and 8199 standards.
Medium for detection and enumeration of lipolytic microorganisms in food stuffs and other materials.
Liquid culture medium for the propagation of ß-haemolytic streptococci and for serological typing studies
Liquid medium for the selective enrichment of Salmonella in the examination of pharmaceutical products
according to the harmonized European Pharmacopoeia methods. This medium needs the addition of
potassium tetrathionate.
To achieve greater selectivity is added to the medium Novobiocin Selective Supplement (Art. No. 06-139LYO1).
This formulation is according to the Arret and Kirschbaum formulation, adopted by US, FDA for the preparation
of spore suspension for antibiotic assay
Liquid medium for enrichment of Yersinia spp. in food samples according ISO 10273 standard.
Solid culture medium used as a basal layer in the enumeration of somatic coliphages in samples of any type
of water, sediment and sludge, according to ISO 10705-2 standard.
Solid medium with high reducing and nutrient capacity for the culture of fastidious anaerobic microorganisms.
Solid culture medium for total enumeration of yeasts and moulds according to ISO 13681. This medium need
to add Oxytetracycline Selective Supplement (Art. No. 06-115LYO1).
Liquid medium to verify the utilitation of rhamnose in fastidious microorganisms, such as Listeria spp.,
according to ISO 11290.
Widely used isotonic diluent in microbiology for maximun recovery of microorganisms formulated according to ISO
6887-1, 8261, 21149, 21150, 22717 and 22718, with neutralizing agents TLH (Polysorbate 80 – Lecithin – Histidine)
Solid selective medium used for the detection and isolation of lactic acid bacteria in the beer and brewing
process
Broth base used for the biochemical differentiation of Shigella spp., based on carbohydrate fermentation
according to ISO 2156
Culture medium for Enterobacteriaceae, according to Ewing et al, for detecting the ability of the Proteus genus to
convert phenylalanine into phenilpiruvic acid. Requires the addition of 0.2 ml of a 10% solution of ferric chloride.
Liquid culture medium, suitable for sugar and other substrate fermentation studies according to ISO 10273
and 22964 standards.
Liquid medium for neutralizing antimicrobials, according to the European Pharmacopoeia. Needs Polysorbate
80 (Art. No. TW0080) additive.
Diluent with neutralisers for the homogenization of samples according to the American Public Health Association.
Widely recommended medium for antibiotic and Sulphonamides susceptibility testing, according to the Kirby-Bauer
and the Ericsson methods.
Solid medium for resuscitation and recovery of Escherichia coli damaged cells by heat, freezing or chemical
process according to FIL-IDF and ISO 11866-2 and 16649-1 standards. Needs ammonium chloride (Art. No.
AM0273) and Sodium Glutamate (Art. No. SO0400) additives.