Irgasan Ticarcillin and Potassium Chlorate Broth (ITC) ISO
Principles and uses
Irgasan Ticarcillin and Potassium Chlorate Broth (ITC) is recommended by ISO 10273 as a selective enrichment broth for the detection of the human pathogenic strain of Yersinia enterocolitica in food and water samples.
Enzymatic casein digest provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group essential for bacterial growth. Magnesium chloride and malachite green, make the broth highly selective. Irgasan inhibits Gram-positive bacteria, Ticarcillin has bactericide on Gram-negative and Gram-positive bacteria and potassium chlorate has a disinfecting property.
Preparation
Suspend 44,0 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121 ºC for 15 minutes. Cool to 47 ºC and aseptically add two vials of ITC Supplement.
Homogenize gently and dispense into sterile containers.