E&O Laboratories Ltd have successfully grown their Culture Media business over the last 30 years to become a leading manufacturer
servicing Microbiology Laboratories , Location in Scotland. Their comprehensive media range is continually expanding into specialised industry sectors as well as aiding the rapid diagnosis of the newest antibiotic resistant bacteria strains.

INDUSTRY SECTORS:
• Clinical, Veterinary & Aquaculture
• Food, Water & Environmental
• Pharmaceutical, Industrial & Cosmetics
• Academia & Research

PRODUCT CATEGORIES:
• Ready-to-use Culture Media – Bottled, Bagged and Plated
• Dehydrated Culture Media & Raw Materials
• Antibiotic Supplements & Reagents
• Fresh Donor Horse & Sheep Blood – Frozen Sterile Filtered Serum

Dehydrated Culture Media products are formulated to supply the required nutrients to allow for the growth of microorganisms. Used in combination with a variety of selective agents and incubation conditions a wide range of specific organisms can be isolated. With careful raw material selection of the various media components E&O can ensure a consistent level of quality and performance. For each formulation the necessary ingredients are accurately weighed, combined and blended together to produce a homogenous powdered product.

Sorbitol Peptone Broth and Bile Salts (PSB) ISO

Principles and uses
Sorbitol Peptone Broth And Bile Salts (PSB) is a medium recommended by ISO 10273 for the selective enrichment of Yersinia enterocolitica in food samples. Outbreaks of gastroenteritis associated with Yersinia enterocolitica are increasing. Contaminated food products such as pork, beef, and raw and processed milk have been identified as sources of infection.
Enzymatic digest of casein provides nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Potassium phosphates act as a buffer system. Sorbitol is the fermentable carbohydrate providing carbon and energy.

Preparation
Suspend 31 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes.

For the selective enrichment of Yersinia enterocolitica.

Enzymatic digest of casein 5 Bile salts 1.5 Sodium chloride 5 Sodium dihidrogenphosphate 1.2 Sodium hydrogen phosphate 8.23 Sorbitol 10

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CIN Supplement (Yersinia Selective Supplement)