E&O Laboratories Ltd have successfully grown their Culture Media business over the last 30 years to become a leading manufacturer
servicing Microbiology Laboratories , Location in Scotland. Their comprehensive media range is continually expanding into specialised industry sectors as well as aiding the rapid diagnosis of the newest antibiotic resistant bacteria strains.

INDUSTRY SECTORS:
• Clinical, Veterinary & Aquaculture
• Food, Water & Environmental
• Pharmaceutical, Industrial & Cosmetics
• Academia & Research

PRODUCT CATEGORIES:
• Ready-to-use Culture Media – Bottled, Bagged and Plated
• Dehydrated Culture Media & Raw Materials
• Antibiotic Supplements & Reagents
• Fresh Donor Horse & Sheep Blood – Frozen Sterile Filtered Serum

Dehydrated Culture Media products are formulated to supply the required nutrients to allow for the growth of microorganisms. Used in combination with a variety of selective agents and incubation conditions a wide range of specific organisms can be isolated. With careful raw material selection of the various media components E&O can ensure a consistent level of quality and performance. For each formulation the necessary ingredients are accurately weighed, combined and blended together to produce a homogenous powdered product.

Trypticasein Soy Broth Modified with Novobiocin (mTSB) ISO

Principles and uses
Trypticasein Soy Broth Modified with Novobiocin (mTSB) is recommended by ISO 16654 for the enrichment of E.coli O157:H7.
Casein peptone and soy peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dipotassium phosphate acts as a buffer system. Glucose is the fermentable carbohydrate providing carbon and energy. Bile salts and Novobiocin are inhibitors of gram-positive organisms.
ISO 16654 recommends preparing the initial suspension and adding a test portion to the 41,5 ºC pre-warmed broth to obtain a ratio of test portion to mTSB + sample of 1/10 (mass to volume, or volume to volume). Incubate for 6 hours then a further 12 hours to 18 hours at 41,5 ºC.

Escherichia coli O157 Selective enrichment

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Modified phosphate buffer (E.coli O157)