E&O Laboratories Ltd have successfully grown their Culture Media business over the last 30 years to become a leading manufacturer
servicing Microbiology Laboratories , Location in Scotland. Their comprehensive media range is continually expanding into specialised industry sectors as well as aiding the rapid diagnosis of the newest antibiotic resistant bacteria strains.

INDUSTRY SECTORS:
• Clinical, Veterinary & Aquaculture
• Food, Water & Environmental
• Pharmaceutical, Industrial & Cosmetics
• Academia & Research

PRODUCT CATEGORIES:
• Ready-to-use Culture Media – Bottled, Bagged and Plated
• Dehydrated Culture Media & Raw Materials
• Antibiotic Supplements & Reagents
• Fresh Donor Horse & Sheep Blood – Frozen Sterile Filtered Serum

Dehydrated Culture Media products are formulated to supply the required nutrients to allow for the growth of microorganisms. Used in combination with a variety of selective agents and incubation conditions a wide range of specific organisms can be isolated. With careful raw material selection of the various media components E&O can ensure a consistent level of quality and performance. For each formulation the necessary ingredients are accurately weighed, combined and blended together to produce a homogenous powdered product.

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O-F Base Medium-image

O-F Base Medium

  • Product Details
  • APPLICATIONS
  • SPECIFICATIONS
  • CODE
  • DOCUMENTS

O-F Medium is used for the determination of oxidative and fermentative metabolism of carbohydrates by Gram-negative bacilli (1).
This is on the basis of the acid reaction in either the open or closed system that has been covered with sterile paraffin oil.
Changes in the covered agar are considered to be due to true fermentation, while changes in the open tubes are due to the oxidative utilization of the carbohydrate present.
O-F Base Medium requires the addition of the specific carbohydrate being investigated.
The enzymatic digest of casein provides the required nitrogen, carbon and vitamins in the media.
Sodium chloride maintains the osmotic balance.
Di-potassium hydrogen phosphate acts as a buffer and bromothymol blue is a pH indicator.
The agar is a solidifying agent.

Reference
(1) Hugh, R. and Leifson, E.J. 1953. Bacteriol. 66:24-26.

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