Môi trường vi sinh ChromoGel™ Listeria Agar Base (ALOA) acc ISO 11290
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ChromoGel™ Listeria Agar completed with selective and enrichment supplements, is a selective and differential medium for the isolation of Listeria spp. from foodstuffs and other samples and for the identification of L. monocytogenes by ISO 11290
Preparation
# Dissolve 70g in 1000ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Autoclave at 121°C for 15 minutes
# Cool to 45-50°C, add 2 vials of ChromoGel Listeria Selective Supplement and 2 vials of ChromoGel Listeria Enrichment Supplement
# Mix well and dispense into appropriate sterile containers
Selective supplement
Formula for 500ml of medium Vial
– Nalidixic Acid 10 mg
– Ceftazidime 10 mg
– Amphotericin B 5 mg
– Polymyxin B 38350 UI
Enrichment supplement
Formula for 500ml of medium Vial
– L-α-Phosphatidylinositol :1g
