Brand: FSTest

CatNO: DR32.5063.5

Specifications: 96tests/kit

Shelf life: 12 months

Application: Designed for fast and precise detection of SEM residues in meat, poultry, fish, shrimp, and seafood products, ensuring food safety and compliance with international regulatory limits.

FSTest Nitrofurazone Metabolite (SEM) ELISA Test Kit – High Sensitivity, Accurate Quantification, and Rapid Analysis

The FSTest Nitrofurazone Metabolite (SEM) ELISA Test Kit is a highly sensitive enzyme-linked immunosorbent assay (ELISA) designed for the quantitative detection of nitrofurazone metabolites (SEM) in animal tissues and seafood.  This test kit offers precise, low-detection limit results, making it an essential tool for food safety testing laboratories, regulatory agencies, and quality control departments.

Key Features & Benefits:

✔ High Sensitivity – Ultra-low detection limit of 0.04ppb, ensuring trace-level residue detection.

✔ Accurate Quantification – Provides precise concentration measurements for regulatory compliance.

✔ Simultaneous Batch Testing – 96-well format allows for high-throughput analysis, improving efficiency.

✔ Reliable & Reproducible – Average coefficient of variation ≤ 10%, ensuring test consistency.

✔ Easy Sample Preparation – Compatible with animal tissue and seafood samples with a simple extraction protocol.

✔ Complies with Global Standards – Ideal for food safety screening in compliance with EU, US, and international regulations.

Applications:

Designed for fast and precise detection of SEM residues in meat, poultry, fish, shrimp, and seafood products, ensuring food safety and compliance with international regulatory limits.

Testing Principle:

This ELISA test uses a competitive immunoassay approach, where SEM in the sample competes with enzyme-conjugated SEM for binding to specific antibodies.  The color intensity measured at 450nm is inversely proportional to the SEM concentration in the sample.  The results are then compared against a standard curve to determine SEM levels accurately.

How to Use:

1️⃣ Sample Preparation – Homogenize and extract the sample following the protocol.

2️⃣ Test Procedure:

Add 50μL of standard or sample to each test well.

Add 50μL of enzyme conjugate solution and 50μL of antibody solution.

Incubate at 20-28°C for 30 minutes in the dark.

Wash the wells 4-5 times and remove excess liquid.

Add 100μL of substrate solution, incubate for 30 minutes, then add 100μL of stop solution.

Measure absorbance at 450nm using a microplate reader.

3️⃣ Result Interpretation – Compare absorbance values with the standard curve to determine SEM concentration.

Why Choose FSTest SEM ELISA Kit?

✅ Enhanced Detection Accuracy – Detects SEM residues down to 0.04ppb, meeting stringent food safety standards.

✅ Optimized for Food Industry & Regulatory Testing – Ideal for food manufacturers, testing labs, and government agencies.

✅ Efficient Sample Analysis – Capable of testing multiple samples in a single run, reducing processing time.

Storage & Stability:

Store at 2-8°C in a sealed container.  Shelf life: 12 months.  Do not freeze.

Ensure food safety compliance with the FSTest Nitrofurazone Metabolite (SEM) ELISA Test Kit – the gold standard for highly sensitive, accurate, and cost-effective food residue screening.