AgarCult Listeria Fraser Broth Base

Preparation
# Dissolve 27.5g in 500ml of distilled water (purified water)
# Heat and frequent stirring to completely dissolve the medium
# Autoclave at 121°C for 15 minutes
# Cool to 45-50°C, add 1 vial of AgarCult Listeria Fraser Supplement
# Mix well and dispense into appropriate sterile containers

Formula g/l
Enzymatic digest of animal tissues 5
Enzymatic digest of casein 5
Beef extract 5
Yeast extract 5
Sodium chloride 20
Disodium hydrogen phosphate anhydrous 9.6
Potassium dihydrogen phosphate 1.35
Aesculin 1
Lithium chloride 3
Total 55
pH: 7.2 ± 0.2 at 250C