Môi trường nuôi cấy vi sinh Buffered Peptone Water (ISO)

Môi trường nuôi cấy vi sinh Buffered Peptone Water (ISO)-image

Môi trường nuôi cấy vi sinh Buffered Peptone Water (ISO)

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Formulated to ISO 6579, Buffered Peptone Water (BPW) is a pre-enrichment medium designed to help sub-lethally damaged Salmonella spp. recover before introducing them into a selective enrichment medium.
Enzymatic digest of casein is the source of the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance in the medium. Di-sodium hydrogen phosphate and potassium di-hydrogen phosphate act as buffers in the medium. This nutritious medium is free from inhibitors and is well buffered to maintain pH 7.0 for the incubation period. Sub-lethal injury to Salmonella spp. occurs in many food processes and this pre-enrichment step greatly increases the chance of their recovery, especially if a low number of cells are present in a sample.

Môi trường vi sinh Buffered peptone water

Suspend 20 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes.