CLOSTRIDIUM PERFRINGENS SUPPLEMENT (TSC)

A sterile selective supplement used for isolation and presumptive identification of Clostridium perfringens, according to ISO 7937 and ISO 14189, and other regulations.

Compositon (g/vial)
D-Cycloserine....................................................... 0.200
Reconstitute the original freeze-dried vial by adding
Sterile Distilled Water...........................................6 ml

Note: each vial is sufficient to supplement 500 ml of medium. TSC
Agar Base.

D-cycloserine selective supplement is added to TSC Agar in order to obtain a final selective medium which has the advantage to simplify the counting of plates with high numbers of colonies because smaller colonies of C.perfringrens are formed.
Sodium metabisulphite and ferric ammonium citrate are used as an indicator of sulphite reduction made by Clostridium perfringens spp. that produce black colonies in TSC agar.

A sterile selective supplement used for isolation and presumptive identification of Clostridium perfringens, according to ISO 7937 and ISO 14189, and other regulations.

D-Cycloserine....................................................... 0.200 Reconstitute the original freeze-dried vial by adding Sterile Distilled Water...........................................6 ml