Pyrethroid Rapid Test Kit

Pyrethroid Rapid Test Kit

Pyrethroid Rapid Test Kit-image

Brand: FSTest

CatNO: PR32.4575.5

Specifications: 10 tests/kit.

Shelf life: 12 months.

Application: Rapid testing of Pyrethroid in fruits, vegetables, tea and other agricultural products.

1. Principle

FSTest Pyrethroid Test Kit is based on sandwich lateral flow immunochromatographic assay, which has a window for the process observation and result reading. The T (test) and a C (control) zone on the testing window are invisible before running the assay. After the treated sample is dropped into the sample hole, the liquid will flow through the surface of test trip laterally and react with the pre-coated recombinant antigens. The intensity of color on the T test line has opposite correspondence with the Pyrethroid concentration in the sample. Meanwhile, the C line is expected to become visible always if sample is applied, which acts as an indicator of valid results. In the way described, the presence of Pyrethroid in the sample can be determined.  

2. Application

Intended to test Pyrethroid in fruits and vegetables.

3. Limit of detection

Efficient Cyhalothrin:0.5 mg/kg

Cyhalothrin:1 mg/kg

Deltamethrin:2 mg/kg

4. Kit composition

Test cassette ×10

Extracting Solution (50 mL) ×1

30 mL sampling bottle ×2

User manual. 

The reagents and appliances may be required but not provided: knives or scissors, micropipettes, scale, timers, vortexers and so on. 

5. Specifications 

10 tests 

6. Sample preparation

6.1. Cut the sample into small pieces (about 0.5 cm×0.5 cm). 

6.2. Weigh 1g of sample into a sampling bottle, mix with 3mL extracting solution. Votex or shake over for 1 minute and stand for 1 minute. If the supernatant is not clear, take 1 mL of liquid andcentrifuge at 4000 rpm for 3 minutes.

7. Procedure & Steps 

7.1. Before use, ensure the cassette is between 20 to 40℃, and put it on a flat surface. 

7.2 Pipette 100 μL of sample solution into a microwell, mix for 3-5 times with pipette, then stand for 3 mins. 

7.3. Pipette 100 μL of sample solution into the sample hole of the cassette. 7.4. Interpret the results after 5-8 min. Results are invalid after 8 min.

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