Saline Peptone Water ISO

Saline Peptone Water (Maximum Recovery Diluent) is an isotonic diluent used for maximum recovery of microorganisms, and for the growth of bacterial cultures, principally marine bacteria.
ISO 6887 recommends this medium as a diluent for the preparation of initial suspension for microbiological samples. The low concentration of peptone does not cause a multiplication of the organisms within 1-2 hours of dilution of the sample. It is also used for carbohydrate fermentation tests in many food and environment studies, amongst others. To determine carbohydrate fermentation patterns, add 1,8 ml of 1% Phenol red to reconstitute the dry medium. After dispensing into test tubes with Durham gas collecting vials for gas detection, sterilize at 121 ºC for 15 minutes. Aseptically add sterile carbohydrate solution (Dextrose) to yield 1% final concentration.
Peptone is the nutrient source of nitrogen, vitamins, amino acids and minerals. Sodium chloride maintains the osmotic balance.