Urea 40% solution

Aqueous urea solution 40%, sterilized by filtration and suitable to be used as an additive in culture media. It is supplied to be used with the dehydrated media Urea Agar according to Christensen and Urea Broth. It must be added to these media after the sterilization and with the media cooled to 50-55ºC.
Once it is added, do not reheat the media because urea is thermolabile and heating allows to its break down and ammonium liberation.