Chủng vi sinh Mirobiologics

Salmonella belongs to the family Enterobacteriaceae, is a gram negative, non-spore forming rod shaped bacillus, and the cause many types of infections from mild gastroenteritis to life threatening typhoid fever. Salmonella can be serotyped according to somatic antigens O (group O: O 2 – O 67), surface Vi, phase I flagellar and phase II flagellar (H types: H-a – H-z81) antigens. The somatic O antigens are heat stable and upon which grouping of the organism is based. The H antigens are heat labile and usually associated with motility. H antigen exists in two phases: phase I and phase II.

Deben Diagnostics Salmonella antisera are liquid products, containing specific agglutinins for each group O antigen, Vi antigen and H antigen, that are used for the serological identification of various Salmonella serotypes according to Kauffman-White classification. The antisera are prepared by hyper-immunizing healthy rabbits with reference strains, heating to 56°C for 30 minutes or using formalin treatment, removing analogous agglutinins by suction, before antiseptic filtration. For the preparation of H antisera, reference strains of the organisms killed by formalin treatment are used as an antigen, and for the preparation of Vi serum, Citrobacter ballerup, a strain that has Vi antigen, is used.

Group O antisera are used for the determination test of Salmonella serotypes using slide agglutination, and H antisera using tube agglutination or slide agglutination.