Môi trường nuôi cấy vi sinh Mannitol Salt Agar

Mannitol Salt Agar is selective medium for the isolation of pathogenic staphylococci. The medium conforms to the requirements of the Harmonised USP/EP/JP. Chapman showed that adding a high level of sodium chloride to Phenol Red Mannitol Agar allowed for the recovery of pathogenic staphylococci. Pathogenic staphylococci produced yellow colonies whereas non-pathogenic staphylococci produced small red colonies.
Pancreatic digest of casein, peptic digest of animal tissue and beef extract provide the required nitrogen, carbon and vitamins. The high level of sodium chloride inhibits most organisms other than staphylococci. Mannitol is a carbohydrate that is fermented by coagulase positive staphylococci. Phenol red is the pH indicator.
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